Elastin antibody (ab21610)

Product Name 

Elastin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Elastin

Immunogen 

Elastins pooled from pig, human, dog chicken, rat and cow.

Reacts with 
(species key)

Hu, Rat, Chk, Cow, Dog, Pig

Specificity 

Recognises elastin.

Tested applications 
(see key)

ELISA, IHC-FoFr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000 - 1/2000.
IHC-Fr: 1/50 - 1/200.
IHC-P: 1/50 - 1/200.
WB: 1/200 - 1/500. Predicted molecular weight: 68 kDa.

In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Extracellular matrix of elastic fibers.

Research areas 

Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Extracellular matrix
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Elastin

Relevance 

Elastin is a major structural protein of tissues such as aorta, which must expand rapidly and recover completely. Elastin chains are cross-linked together into an extensible 3D network. It is a molecular determinant of late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Elastin forms a complex with BGN and MFAP2.
Defects in elastin are a cause of autosomal dominant cutis laxa. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience leading to a premature aged appearance. This may be accompanied by pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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