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Anti-Elastin antibody (ab21610)

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Overview

Product name

Anti-Elastin antibody
See all Elastin products (8) ...

Description

Rabbit polyclonal to Elastin

Specificity

Recognises elastin.

Tested applications

IHC-FoFr, IHC-P, ELISA, WBmore details

Cross reactivity

Reacts with

Rat, Chicken, Cow, Dog, Human, Pig

Immunogen

Elastins pooled from pig, human, dog chicken, rat and cow.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum.

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Elastin antibody (ab21610)Western blot - Elastin antibody (ab21610) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab21610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-FoFr

     IHC-FoFr: 1/50 - 1/100.

    IHC-FoFr: 1/50 - 1/100.

  • ShowHide

    IHC-P

     IHC-P: 1/50 - 1/100.(In ...Read more →

    IHC-P: 1/50 - 1/100.(In many cases, antigenicity of elastic fiber components can be enhanced by treating with a 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 solution for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.)

  • ELISA

     ELISA: 1/1000 - 1/2000.

    ELISA: 1/1000 - 1/2000.

  • ShowHide1 Image

    WB

     WB: 1/200 - 1/500.Detect...Read more →

    WB: 1/200 - 1/500.Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

Function

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.

Tissue specificity

Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.

Involvement in disease

Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

Sequence similarities

Belongs to the elastin family.

Post-translational
modifications

Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

Cellular localization

Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.

Target information above from: UniProt accessionP15502 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Elastin antibody
  • ELN antibody
  • ELN_HUMAN antibody
  • FLJ38671 antibody
  • FLJ43523 antibody
  • Supravalvular aortic stenosis antibody
  • SVAS antibody
  • Tropoelastin antibody
  • WBS antibody
  • Williams Beuren syndrome antibody
  • Williams syndrome region antibody
  • WS antibody
see all

Anti-Elastin antibody images:

  Western blot - Elastin antibody (ab21610)

Western blot - Elastin antibody (ab21610)

Anti-Elastin antibody (ab21610) at 1/500 dilution + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 28 kDa,43 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

References for Anti-Elastin antibody (ab21610)

This product has been referenced in:

  • Dodd Tet al. MMPs 2 and 9 are essential for coronary collateral growth and are prominently regulated by p38 MAPK. J Mol Cell Cardiol : (2011). WB; Rat.Read more (PubMed: 21884701) »
  • Paunovic Bet al. Molecular mechanisms of basic fibroblast growth factor effect on healing of ulcerative colitis in rats. J Pharmacol Exp Ther 339:430-7 (2011). Rat.Read more (PubMed: 21841041) »

See all 6 publications for this product

Publishing research using ab21610? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"