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Product Name
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Endogenous Avidin/Biotin Blocking Kit
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Product type
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Kits
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Description
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Endogenous Avidin/Biotin Blocking Kit
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Research areas
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Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
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Product overview
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In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.
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Components
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| Kit Components | Unit Size/Volume |
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| Avidin Block(ready-to-use) | 15ml | | Biotin Block(ready-to-use) | 15ml |
Note:Please Click Here for Instructions For Use/Protocol
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Storage instructions
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Store at +4°C.
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Tested applications
(see key)
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IHC-P
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Material safety datasheet (MSDS) for this product:
Endogenous Avidin/Biotin Blocking Kit MSDS
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Relevance
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Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Endogenous Avidin/Biotin Blocking Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Endogenous Avidin+Biotin Blocking System
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Endogenous Avidin/Biotin Blocking Kit - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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