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Endogenous Avidin/Biotin Blocking Kit (ab64212)

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    Overview

    Product name

    Endogenous Avidin/Biotin Blocking Kit
    See all Endogenous Avidin+Biotin Blocking System products (2) ...

    Product overview

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

    Notes

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

    Tested applications

    IHC-Pmore details

    Properties

    Storage instructions

    Store at +4°C.

    Storage buffer

    Preservative: 0.08% Sodium azide
    Constituent: Avidin

    Components 15 ml
    Avidin Block1 x 15ml
    Biotin Block1 x 15ml

    Research areas

    Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents

    Relevance

    Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab64212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Protocols

  • Endogenous Avidin/Biotin Blocking Kit

    • References for Endogenous Avidin/Biotin Blocking Kit (ab64212)

    ab64212 has not yet been referenced specifically in any publications.

    Publishing research using ab64212? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"