You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Endogenous Avidin + Biotin Blocking System (ab3387)

Q&A (9)Specific References (1)

Overview

  • Product nameEndogenous Avidin + Biotin Blocking SystemSee all Endogenous Avidin+Biotin Blocking System kits ...
  • Notes

    The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin / steptavidin-biotin (ABC systems). Non-specific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

  • Tested applicationsIHC-Fr, IHC-P, ICC/IF more details

Properties

  • RelevanceSome cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

    • Applications

    Our Abpromise guarantee covers the use of ab3387 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-Fr IHC-Fr
    IHC-P IHC-P
    ICC/IF ICC/IF

    References for Endogenous Avidin + Biotin Blocking System (ab3387)

    This product has been referenced in:
    • Chou S & Lodish HF Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells. Proc Natl Acad Sci U S A 107:7799-804 (2010). Read more (PubMed: 20385801) »

    See 1 Publication for this product

    Publishing research using ab3387 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab3387 Submit a Question for ab3387

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

    10
    Votes: Highest First

    Quisiera hacer una pregunta acerca del complejo Avidin Biotin Abcam, la referencia es ab3387, mi pregunta es acerca de la caducidad, no he sabido ver límite de utilización ni en el producto en sí, ni en la página web Abcam.

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the an...

    Read More

    I forgot to mention that we do a serum block before the primary antibody is applied.  We have an avidin-biotin blocking kit that we have tried with no effect on our results.  In our experience, we only need to use the avidin-biotin block in conjunction of...

    Read More

    Thank you for your reply.

    I would suggest changing your antigen retrieval method next. While you have used sodium citrate pH 6 and Tris/EDTA pH 10 I would recommend the following procedure based on a paper from Jiao et al which I will include. <...

    Read More

    My lab has been having some IHC-P issues for a few weeks now and I was wondering if anyone on your team had an insight into our problem.   We use rhesus macaque brain (amongst other) tissue, and have been using the same samples for a few years. They...

    Read More

    Thank you for contacting us.

    I am sorry to hear of the troubles which you have been experiencing in IHC-P. In reviewing the protocol and troubleshooting provided I did not see that you block endogenous avidin or biotin. We highly recommend block...

    Read More

    The attached MSDS does not include the 1st page.  Please send me a complete MSDS for product number ab3387.  

    Thank you for contacting us regarding this matter.

    We have updated the MSDS and have fixed this issue. We would like to thank you for bringing this to our attention. I gave included the updated MSDS here but it is available on the webpage for thi...

    Read More

    Thanks for the reply.

    We never did in our laboratory this protocol with Egg white and milk. Could you explain in more detail how and in what part of the procedure should I enter them.

    I'm very pleased with the attention and speed tha...

    Read More

    Thank you for your inquiry. My colleague is not in the office today and I am happy to answer your question instead of her. Please find below a protocol for blocking biotin with egg white:
    1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has ...

    Read More

    I made more tests and still gives unexpected results, osteoblasts were labeled and not all osteoclasts were labeled.

    I tried two new forms of antigen retrieval:

    1- used skimmed milk to block the endogenous biotin

    2- used b...

    Read More

    Thank you for contacting us.
    Please be aware that the biotin problem is still not solved by using milk or BSA: those two will block the non-specific protein binding sites, but not the endogenous biotin. You can block the endogenous biotin by using ei...

    Read More

    I am having trouble with Ki67 antibody (cat#ab15580). I used it in mouse tissues such as kidney, liver, testis, brain, cartilage etc. fixed in Bouin’s fixative and formaldehyde. The staining was with extremely high background. I tried 1:100 dilution...

    Read More

    Thank you for contacting us.

    Is the UV block from DAKO a protein block? We typically recommend performing a protein block using 10% normal serum from the host species of your secondary antibody. For example, if your secondary is goat anti-rabbit...

    Read More

    Good morning, I'm currently using Abcam products to perform imunohistochemistry on paraffinon my research protocols and I have some doubts that I would likeAbcam tohelp me. I worked with Anti-Survivin antibody (ab469) as a primary antibody in a 1/500 dilu...

    Read More

    Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully underst...

    Read More

    LOT NUMBER GR53220 ORDER NUMBER 975372 DESCRIPTION OF THE PROBLEM No staining SAMPLE mouse: lung, pancreas, CT26-Tumor, LPS-liver PRIMARY ANTIBODY 1:25 and 1:50 in PBS incubation overnight at roomtemperature DETECTION METHOD Steaptavidin-POD, 1:50, 10 min...

    Read More

    Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Es tut mir leid, dass Sie Probleme mit unserem VEGF-Antikörper ab3109 hatten. Ich weiß, dass Sie schon viel Zeit in Ihre Experimente investie...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"