|
|
|
|
|
Product Name
|
Endogenous Avidin + Biotin Blocking System
|
|
Product type
|
IHC reagents
|
|
Description
|
Endogenous Avidin + Biotin Blocking System
|
|
Tested applications
(see key)
|
ICC, IHC-Fr, IHC-P, WB
|
|
Application notes
(see key)
|
Recommended dilutions
Protocols differ considerably from lab to lab, therefore incorporate the blocking step at the point which best corresponds to the following:
1. Deparaffinize specimens.
2. Block with peroxide.
3. Perform Heat Induced Epitope Retrieval [if needed].
4. Block with serum block.
5. Block with avidin / biotin blocking solutions:
5.1 Incubate the avidin Solution for 15 minutes at RT.
5.2 Rinse with PBS or TBS buffer for 3-4 times.
5.3 Incubate with the biotin Solution for 15 minutes.
5.4 Rinse with PBS or TBS Buffer for 3-4 times.
6. Apply the primary antibody.
7. Apply the biotinylated secondary antibody.
8. Apply the streptavidin/HRP conjugate.
9. Apply the chromogen.
10. Counter-Stain.
11. Coverslip.
TROUBLESHOOTING:
Always use these components in sequence. First avidin, then biotin.
If you dilute these products or use them as a buffer for other components of your assay,dilute them proportionately and use them in the correct sequence.
If you have weakened specific staining try shortening the incubation time of the avidin and biotin components.
Non-specific IgG present in polyclonal anti-sera can also create non-specific staining.
|
|
Research areas
|
Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
|
|
|
|
|
Relevance
|
Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.
|
|
Form
|
This product consists of 2 separate vials in a ready-to-use format:
1x 15 ml vial of avidin solution.
1x 15 ml vial of biotin solution.
|
|
Storage instructions
|
Store at +4°C.
|
|
Notes
|
The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin / steptavidin-biotin (ABC systems). Non-specific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Endogenous Avidin + Biotin Blocking System is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to Endogenous Avidin+Biotin Blocking System
|
| |
Endogenous Avidin + Biotin Blocking System - more information
|
Customer reviews (feedback) regarding Endogenous Avidin + Biotin Blocking System |
Customer FAQs regarding Endogenous Avidin + Biotin Blocking System |
Protocols for Endogenous Avidin + Biotin Blocking System |
Price and availability of products related to Endogenous Avidin + Biotin Blocking System |
| |
Endogenous Avidin + Biotin Blocking System - other tools:
|
Contact Abcam with a Technical Enquiry about Endogenous Avidin + Biotin Blocking System |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
