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Endogenous Avidin + Biotin Blocking System (ab3387)

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Overview

Product name

Endogenous Avidin + Biotin Blocking System
See all Endogenous Avidin+Biotin Blocking System products (2) ...

Notes

The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin / steptavidin-biotin (ABC systems). Non-specific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

Tested applications

IHC-Fr, IHC-P, ICC/IFmore details

Properties

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide in both components

Components 1 kit
Avidin solution1 x 15ml
Biotin Solution1 x 15ml

Research areas

Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents

Relevance

Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Protocols

  • Endogenous Avidin + Biotin Blocking System

    • References for Endogenous Avidin + Biotin Blocking System (ab3387)

    This product has been referenced in:

    • Chou S & Lodish HF Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells. Proc Natl Acad Sci U S A 107:7799-804 (2010).Read more (PubMed: 20385801) »

    See 1 publication for this product

    Publishing research using ab3387? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"