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Product name
Anti-Endostatin antibody [91318]
See all Endostatin products (14) ...
Description
Rat monoclonal [91318] to Endostatin
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Purified recombinant mouse endostatin, expressed in insect Sf 21 cells.
General notes
Endotoxin level is <10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The antibody is purified from the IgG fraction of ascities fluid using protein G.
Clonality
Monoclonal
Clone number
91318
Isotype
IgG2b
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Cardiovascular >> Angiogenesis >> Inhibitors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse endostatin is approximately 6 ng/well.
WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant mouse endostatin is approximately 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Predicted molecular weight: 22 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Tissue specificity
Present in multiple organs with highest levels in liver, lung and kidney.
Involvement in disease
Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
Sequence similarities
Belongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Post-translational
modifications
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP39060
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha 1 Collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
see all
Database links
- Entrez Gene: 12819 Mouse
- Entrez Gene: 12822 Mouse
- SwissProt: O35206 Mouse
- SwissProt: P39061 Mouse
- Unigene: 233547 Mouse
- Unigene: 4352 Mouse
References for Anti-Endostatin antibody [91318] (ab10604)
ab10604 has not yet been referenced specifically in any publications.
Publishing research using ab10604? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"