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ab58821 staining mouse Endothelial cells derived from heart tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Heart tissue was disassociated by the collagenase/trypsin method and the isolated cells were cultured on a chamber slide with DMEM/FCS medium. Cells were fixed with ice cold acetone and blocked with 5% BSA for 1 hour at 4ºC. Samples were incubated with primary antibody 1/100 in PBS for 12 hours at 4ºC. An Alexa Fluor® 555-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained by DAPI.
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