You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Endothelial Cell antibody [A10-33/1] (ab58821)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Endothelial Cell antibody [A10-33/1]
See all Endothelial Cell products (4) ...

Description

Mouse monoclonal [A10-33/1] to Endothelial Cell

Specificity

Stains preferentially large vessels of the liver, lung capillaries, gall bladder and skin. Generally reactive with melanoma, including metastasizing melanomas and in carcinomas. Cross reactivity with nervous tissue has been reported.

Tested applications

ICC/IF, WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Cow, Human

Immunogen

Melanoma cell lines A-375 and SK-Mel 25, and subclone S-5 of SK-Mel 25.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

FPLC purified

Clonality

Monoclonal

Clone number

A10-33/1

Isotype

IgG1

Research areas

Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial

  • Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or IHC-P.

Target

Relevance

The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

Anti-Endothelial Cell antibody [A10-33/1] images:

  Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)

Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)

ab58821 staining mouse Endothelial cells derived from heart tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Heart tissue was disassociated by the collagenase/trypsin method and the isolated cells were cultured on a chamber slide with DMEM/FCS medium. Cells were fixed with ice cold acetone and blocked with 5% BSA for 1 hour at 4°C. Samples were incubated with primary antibody 1/100 in PBS for 12 hours at 4°C. An Alexa Fluor® 555-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained by DAPI.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Endothelial Cell antibody [A10-33/1] (ab58821)

ab58821 has not yet been referenced specifically in any publications.

Publishing research using ab58821? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"