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Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)
![Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)](/ps/datasheet/images/58/ab58821/Endothelial-Cell-Primary-antibodies-ab58821-3.jpg)
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Product Name
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Endothelial Cell antibody [A10-33/1]
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See all Endothelial Cell antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [A10-33/1] to Endothelial Cell
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Immunogen
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Melanoma cell lines A-375 and SK-Mel 25, and subclone S-5 of SK-Mel 25.
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Reacts with
(species key)
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Hu, Ms, Cow
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Specificity
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Stains preferentially large vessels of the liver, lung capillaries, gall bladder and skin. Generally reactive with melanoma, including metastasizing melanomas and in carcinomas. Cross reactivity with nervous tissue has been reported.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100.
IHC-Fr: 1/100 - 1/400.
WB: Use at an assay dependent dilution.
Is unsuitable for IHC-P. The antigen is sensitive to formaldehyde.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
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Relevance
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The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary.
Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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A10-33/1
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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FPLC purified
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Endothelial Cell antibody [A10-33/1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Endothelial Cell antibody [A10-33/1] images, references, products related to ab58821 and other tools.
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Endothelial Cell antibody [A10-33/1] images:
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 Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)
ab58821 staining mouse Endothelial cells derived from heart tissue by ICC/IF (Immunocytochemistry/immunoflurescence). Heart tissue was disassociated by the collagenase/trypsin method and the isolated cells were cultured on a chamber slide with DMEM/FCS medium. Cells were fixed with ice cold acetone and blocked with 5% BSA for 1 hour at 4°C. Samples were incubated with primary antibody 1/100 in PBS for 12 hours at 4°C. An Alexa Fluor® 555-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained by DAPI. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Endothelial Cell
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Endothelial Cell antibody [A10-33/1] - more information
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Customer reviews (feedback) regarding Endothelial Cell antibody [A10-33/1] |
Customer FAQs regarding Endothelial Cell antibody [A10-33/1] |
Protocols for Endothelial Cell antibody [A10-33/1] |
Price and availability of products related to Endothelial Cell antibody [A10-33/1] |
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Endothelial Cell antibody [A10-33/1] - related products:
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Endothelial Cell antibody [A10-33/1] - other tools:
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Contact Abcam with a Technical Enquiry about Endothelial Cell antibody [A10-33/1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [A10-33/1] (ab58821)](/index.html?datasheet=58821#Endothelial-Cell-Primary-antibodies-ab58821-3.jpg)
