Endothelial Cell antibody [PAL-E] (ab8853)

Product Name 

Endothelial Cell antibody [PAL-E]

Product type 

Primary antibodies

Description 

Mouse monoclonal [PAL-E] to Endothelial Cell

Immunogen 

Purified endothelium.

Reacts with 

Hu, Goat, Pig, Rb

Does not react with 
(species key)

Ms, Rat, Chk

Specificity 

This antibody is specific against human endothelial cells.

Tested applications 
(see key)

IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1:50-1:100. It is recommended that this product be used on acetone-fixed frozen tissue sections. For a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. The optimal conditions should be determined by the individual laboratory.

This antibody is not compatible with formalin-fixed, paraffin-embedded sections. Not suitable for cell suspensions or western blots.

Positive control 
(see definition)

Tonsil, spleen.

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial

Relevance 

The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary.
Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.




This antibody reacts with endothelium on human blood vessels with the exception of arteries. It cross-reacts with goat, rabbit and pig endothelia. Lymphatic endothelium, histiocytes and megakaryocytes are not reactive with this antibody.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

PAL-E

Isotype 

IgG2a

Purity 

Tissue culture supernatant

Storage buffer 

20 mM tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.1% sodium azide, pH 7.5.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.400 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

References for Endothelial Cell antibody [PAL-E] (ab8853)

  This product has been used in: (two most recent references)

Niemela H et al. Molecular identification of PAL-E, a widely used endothelial-cell marker. Blood 106:3405-9 (2005). PubMed: 16099878

Jones RR et al. Histogenesis of Kaposi's sarcoma in patients with and without acquired immune deficiency syndrome (AIDS). J Clin Pathol 39:742-9 (1986). PubMed: 3090109

See all 4 publication references for this product.


If you publish research using ab8853 please let us know so that we can cite the reference on this datasheet.

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