Anti-Endothelial Cell antibody [RECA-1] (ab9774)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (ab9774)

IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  Immunocytochemistry/ Immunofluorescence - Endothelial Cell antibody [RECA-1] (ab9774)

ab9774 staining cells of rat liver sections by ICC/IF.  Cells were acetone fixed and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab9774 diluted 1/25 for 30 minutes at 25°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody was used as the secondary.

Double IIF labeling with RECA-1 (Green) and dipeptidyl peptidase IV (Red)

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  Immunohistochemistry (Frozen sections) - Endothelial Cell antibody [RECA-1] (ab9774)

ab9774 staining endothelial cells in Rat lung tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 100% SuperBlock Blocking Buffer for 20 minutess at 23°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody, diluted 1/200.

This image is courtesy of an anonymous Abreview.

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