Endothelial Cell antibody [RECA-1] (ab9774)

Product Name 

Endothelial Cell antibody [RECA-1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [RECA-1] to Endothelial Cell

Immunogen 

Peripheral and mesenteric lymph nodes from AO rat

Reacts with 
(species key)

Rat

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-G, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
IF: 1/10 - 1/50. Fix tissue in 2% paraformaldehyde in PBS (pH 7.4) for 3 hours and maintain overnight in 6% sucrose in PBS, rinse with acetone, and embed in Technovit 8100 (glycol methacrylate). Do not perform antigen retrieval (see Duijvestejn AM et al. for details).
IHC-P: 1/500 (PMID 19088740).
IHC-Fr: 1/50.
IHC-G: 1/10 - 1/50.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial

Relevance 

The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary.
Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.




RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

RECA-1

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS with 0.1% BSA 0.02% sodium azide pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Endothelial Cell antibody [RECA-1] (ab9774)

  This product has been used in: (two most recent references)

Park E et al. An analysis of regional microvascular loss and recovery following two grades of fluid percussion trauma: a role for hypoxia-inducible factors in traumatic brain injury. J Cereb Blood Flow Metab 29:575-84 (2009). IHC-P; Rat. PubMed: 19088740

Kong D et al. Cytokine-induced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries. Circulation 110:2039-46 (2004). PubMed: 15451799

See all 4 publication references for this product.


If you publish research using ab9774 please let us know so that we can cite the reference on this datasheet.

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