Endothelin 1 293T Transfected Lysate - (positive control) (ab94258)

Overview

  • Product nameEndothelin 1 293T Transfected Lysate - (positive control)
  • General notesab94258 is a 293T cell transfected lysate in which Human Endothelin 1 has been transiently over-expressed using a pCMV-Endothelin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Endothelins are endothelium-derived vasoconstrictor peptides. Tissue specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Similarity: Belongs to the endothelin/sarafotoxin family.

Applications

Our Abpromise guarantee covers the use of ab94258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Endothelin 1 293T Transfected Lysate - (positive control) images

    • ab94258 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-Endothelin 1 antibody (ab88093) at 1/500 dilution

      Lane 1 : Endothelin 1 293T Transfected Lysate - (positive control) (ab94258)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Endothelin 1 293T Transfected Lysate - (positive control) (ab94258)

    ab94258 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"