Anti-Endothelin A Receptor/ET-A antibody (ab117521)
Key features and details
- Rabbit polyclonal to Endothelin A Receptor/ET-A
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Endothelin A Receptor/ET-A antibody
See all Endothelin A Receptor/ET-A primary antibodies -
Description
Rabbit polyclonal to Endothelin A Receptor/ET-A -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Mouse Endothelin A Receptor/ET-A aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q61614 -
Positive control
- Mouse dorsal root ganglia; Mouse heart tissue; Endothelin A Receptor/ET-A transfected cells; Mouse heart lysate
-
General notes
We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
1/5000.
|
|
WB |
1/5000. Predicted molecular weight: 49 kDa.
|
|
IHC-P | (1) |
1/2000.
|
Notes |
---|
ICC
1/5000. |
WB
1/5000. Predicted molecular weight: 49 kDa. |
IHC-P
1/2000. |
Target
-
Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 281749 Cow
- Entrez Gene: 450187 Dog
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 397457 Pig
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- Omim: 131243 Human
see all -
Alternative names
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
Images
-
ab117521, at 1/5000 dilution, staining Endothelin A Receptor/ET-A in HEK293 transfected with Endothelin A receptor cells by Immunocytochemistry.
-
ab117251, at 1/2000 dilution, staining Endothelin A Receptor/ET-A in the atrium of mouse heart by Immunohistochemistry.
-
Anti-Endothelin A Receptor/ET-A antibody (ab117521) at 1/5000 dilution + Mouse heart lysate
Predicted band size: 49 kDa -
Immunohistochemistry analysis of mouse dorsal root ganglia labeling Endothelin A Receptor/ET-A with ab117521.
Protocols
Datasheets and documents
-
Datasheet download
References (16)
ab117521 has been referenced in 16 publications.
- Lee YJ et al. The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation. Int J Oncol 62:N/A (2023). PubMed: 36453252
- Feng L et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
- Au M et al. Endothelin-1 induces chondrocyte senescence and cartilage damage via endothelin receptor type B in a post-traumatic osteoarthritis mouse model. Osteoarthritis Cartilage 28:1559-1571 (2020). PubMed: 32858189
- Adams KL et al. Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone. Nat Commun 11:2138 (2020). PubMed: 32358570
- Kong H et al. Endothelin receptors promote schistosomiasis-induced hepatic fibrosis via splenic B cells. PLoS Pathog 16:e1008947 (2020). PubMed: 33075079