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Anti-Engrailed and Invected antibody [4D9] (ab12454)

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Overview

Product name

Anti-Engrailed and Invected antibody [4D9]

Description

Mouse monoclonal [4D9] to Engrailed and Invected

Specificity

This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.

Tested applications

IHC-FoFrmore details

Cross reactivity

Reacts with

Chicken, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish, Crayfish

Does not react with

Mouse

Immunogen

C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

Epitope

Epitope has been localized to residues 38-58 of the homeodomain.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

4D9

Myeloma

NS1

Isotype

IgG1

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

  • Immunocytochemistry - Anti-Engrailed and Invected antibody [4D9] (ab12454)Immunocytochemistry - Anti-Engrailed and Invected antibody [4D9] (ab12454) image (enlarge)

  • Immunofluorescence - Engrailed and Invected antibody [4D9] (ab12454)Immunofluorescence - Engrailed and Invected antibody [4D9] (ab12454) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-FoFr

     IHC-FoFr: 1/200(Fix with...Read more →

    IHC-FoFr: 1/200(Fix with 4% paraformaldehyde in PBS for 1 hour.)

Target

Relevance

In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.

Alternative names

  • En antibody
  • Eng antibody
  • Engrailed antibody
  • Engrailed/Invected antibody
  • Homeobox protein invected antibody
  • Inv antibody
  • Invected antibody
see all

Database links

Anti-Engrailed and Invected antibody [4D9] images:

  Immunocytochemistry - Anti-Engrailed and Invected antibody [4D9] (ab12454)

Immunocytochemistry - Anti-Engrailed and Invected antibody [4D9] (ab12454)

ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.Visualisation was with DAB.

  Immunofluorescence - Engrailed and Invected antibody [4D9] (ab12454)

Immunofluorescence - Engrailed and Invected antibody [4D9] (ab12454)

ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

Protocols

  • Recommended IHC protocol with ab12454

    • References for Anti-Engrailed and Invected antibody [4D9] (ab12454)

    This product has been referenced in:

    • Baratte Set al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007).Read more (PubMed: 17394016) »
    • Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003).Read more (PubMed: 12781681) »

    See all 11 publications for this product

    Publishing research using ab12454? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"