Engrailed and Invected antibody [4D9] (ab12454)

Product Name 

Engrailed and Invected antibody [4D9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4D9] to Engrailed and Invected

Immunogen 

C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

Reacts with 

Chk, Cyfsh, Dm, Xl, Zfsh

Does not react with 
(species key)

Ms

Specificity 

This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.

Epitope 

Epitope has been localized to residues 38-58 of the homeodomain.

Tested applications 
(see key)

IHC-FoFr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-F: 1/200. ab12454 can be used with embryos fixed for 1 hour in phosphate-buffered 4% paraformaldehyde (pH 7.0) (Rowan et al.). Antigen retrieval is not normally necessary.

The antibody will recognize denatured proteins and is also valuable for expression cloning.



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor.
Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4D9

Myeloma 

NS1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Engrailed and Invected antibody [4D9] images, references, products related to ab12454 and other tools.

Engrailed and Invected antibody [4D9] images:

References for Engrailed and Invected antibody [4D9] (ab12454)

  This product has been used in: (two most recent references)

Baratte S et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). PubMed: 17394016

Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). PubMed: 12781681

See all 11 publication references for this product.


If you publish research using ab12454 please let us know so that we can cite the reference on this datasheet.

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