Anti-EVPL antibody (ab204237)
Key features and details
- Rabbit polyclonal to EVPL
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EVPL antibody
See all EVPL primary antibodies -
Description
Rabbit polyclonal to EVPL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human EVPL aa 100-250.
Database link: Q92817 -
Positive control
- IHC-P: Human skin tissue; ICC: MCF7 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
EVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes. -
Cellular localization
Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments. -
Database links
- Entrez Gene: 2125 Human
- Entrez Gene: 14027 Mouse
- Entrez Gene: 303687 Rat
- Omim: 601590 Human
- SwissProt: Q92817 Human
- SwissProt: Q9D952 Mouse
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Alternative names
- 210 kDa cornified envelope antibody
- 210 kDa cornified envelope precursor protein antibody
- 210 kDa paraneoplastic pemphigus antigen antibody
see all
Images
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Immunohistochemical analysis of human skin tissue labeling EVPL (high expression) with ab204237 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling EVPL in the intermediate filaments with ab204237 at 2 µg/ml.
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Immunohistochemical analysis of human skeletal muscle tissue labeling EVPL (low expression) with ab204237 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204237 has not yet been referenced specifically in any publications.