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ab8666 has been referenced in 7 publications.
Publishing research using ab8666? Please let us know so that we can cite the reference in this datasheet
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Histogram plot of prostate tumour cells DU145 (green) over isotype control (red) stained with EpCam following resection.
This picture was kindly supplied as part of the review submitted by Phil Kearney.
ab8666 staining EpCAM in murine xenograft tumour derived from human DCIS cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were deparaffinized in xylene, and rehydrated in graded alcohols. Sections were then incubated at room temperature with ab8666 at 5 µg/ml. Slides were rinsed in borate buffer pH 8 and counterstained. EpCAM-FITC pseudo-colored green, 488 nm and DAPI psuedo-colored blue, 408 nm.
Image from Espina V et al, PLoS One. 2010 Apr 20;5(4):e10240, Fig 1.
ICC/IF image of ab8666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8666, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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