EpCAM antibody [B302 (323/A3)] (ab8601)

Product Name 

EpCAM antibody [B302 (323/A3)]

Product type 

Primary antibodies

Description 

Mouse monoclonal [B302 (323/A3)] to EpCAM

Immunogen 

MCF-7 human breast cancer cells

Reacts with 
(species key)

Hu

Specificity 

This antibody stains most epithelial cells and carcinomas.
This antibody is a 40 kD epithelial protein expressed on baso-lateral cell surfaces in many epithelial tissue, but absent on mesothelial tissues.
This transmembrane protein consists of 342 AA, has 3 glycosylation sites and is a homophyllic cell-cell adhesion molecule.

Tested applications 
(see key)

IHC-FoFr, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
This antibody can be used on formalin-fixed, paraffin-embedded and frozen tissue sections at a dilution of 1:50-1:60
When using paraffin-embedded and formalin-fixed tissues, heat mediated antigen retrieval is strongly recommended for consistent and reproducible results
For western blotting use at 1:100-1:300.
The optimal conditions should be determined by the individual laboratory

Positive control 
(see definition)

Human stomach, colon carcinoma.

Cellular localization 

Cell Membrane; single pass type I membrane protein.

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B302 (323/A3)

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

20 mM tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.1% sodium azide, pH 7.5.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.300 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

Notes 

This antibody is also called Epithelial Cellular Adhesion Molecule (EP-CAM) and Epithelial Specific Antigen (EAS)
Prolonged fixation in buffered formalin can destroy the epitope

References for EpCAM antibody [B302 (323/A3)] (ab8601)

  This product has been used in: (two most recent references)

Sivagnanam M et al. Further evidence for EpCAM as the gene for congenital tufting enteropathy. Am J Med Genet A 152A:222-4 (2010). IHC-P; Human. PubMed: 20034091

Shim HS et al. Prognostic significance of paired epithelial cell adhesion molecule and E-cadherin in ovarian serous carcinoma. Hum Pathol 40:693-8 (2009). PubMed: 19157508

See all 5 publication references for this product.


If you publish research using ab8601 please let us know so that we can cite the reference on this datasheet.

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