|
|
|
|
Western blot - EpCAM antibody [E144] (ab32392)
![Western blot - EpCAM antibody [E144] (ab32392)](/ps/datasheet/images/ab32392_1.jpg)
(enlarge)
|
|
Immunohistochemistry (Paraffin-embedded sections) - EpCAM antibody [E144] (ab32392)
![Immunohistochemistry (Paraffin-embedded sections) - EpCAM antibody [E144] (ab32392)](/ps/datasheet/images/ab32392_2.jpg)
(enlarge)
|
|
Western blot - EpCAM antibody [E144] (ab32392)
(enlarge)
|
|
|
|
|
Product Name
|
EpCAM antibody [E144]
|
| |
See all EpCAM antibodies (18)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit monoclonal [E144] to EpCAM
|
|
Immunogen
|
Synthetic peptide corresponding to residues in the C-terminus of Human EpCAM.
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
This antibody is specific for human EpCAM.
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC: 1/100.
ICC/IF: 1/100.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/2500. Predicted molecular weight: 39 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
A431 cell lysate and stomach carcinoma.
|
|
Cellular localization
|
Cell Membrane; single pass type I membrane protein.
|
|
Research areas
|
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
|
|
Relevance
|
Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
E144
|
|
Isotype
|
IgG
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
|
Notes
|
Produced under U.S. Patent No. 5,675,063.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EpCAM antibody [E144] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for EpCAM antibody [E144] images, references, products related to ab32392 and other tools.
|
| |
EpCAM antibody [E144] images:
|
 Western blot - EpCAM antibody [E144] (ab32392)
EpCAM antibody [E144] (ab32392) at 1/2500 dilution + A431 cell lysate.
Predicted band size : 39 kDa
Observed band size : 39 kDa
|
| |
Immunohistochemistry (Paraffin-embedded sections) - EpCAM antibody [E144] (ab32392)
ab32392 at a 1:100 dilution staining EpCAM in stomach carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
|
| |
Western blot - EpCAM antibody [E144] (ab32392)
All lanes : EpCAM antibody [E144] (ab32392) at 1/1000 dilution
Lane 1 : A431 cell line, whole cell lysate, 250,000 cells
Lane 2 : A549 cell line, whole cell lysate, 250,000 cells
Lane 3 : HCT116 cell line, whole cell lysate, 250,000 cells
Lane 4 : HEK293 cell line, whole cell lysate, 250,000 cells
Lane 5 : HeLa cell line, whole cell lysate, 250,000 cells
Lane 6 : MCF7 cell line, whole cell lysate, 250,000 cells
Lane 7 : MDA-MB-231 cell line, whole cell lysate, 250,000 cells
Lane 8 : UPCI:OSCC40 cell line, whole cell lysate, 250,000 cells
Secondary
HRP-conjugated donkey anti-rabbit IgG whole antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 39 kDa
Observed band size : 39 kDa
Exposure time : 5 minutes
The membrane was blocked with 5% milk for 18 hours at 4°C. This image is courtesy of an Abreview submitted by Ms Abigale Lade
See Abreview
|
| |
Immunocytochemistry/ Immunofluorescence-EpCAM antibody [E144](ab32392)
](\ps\datasheet\images\32\ab32392\EpCAM-Primary-antibodies-ab32392-1.jpg)
ICC/IF image of ab32392 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32392, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
References for EpCAM antibody [E144] (ab32392)
|
|
This product has been used in:
Tan MC et al. Disruption of CCR5-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer. J Immunol 182:1746-55 (2009). IHC-P; Human. PubMed: 19155524
If you publish research using ab32392 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to EpCAM
|
| |
EpCAM antibody [E144] - more information
|
Customer reviews (feedback) regarding EpCAM antibody [E144] |
Customer FAQs regarding EpCAM antibody [E144] |
Protocols for EpCAM antibody [E144] |
Price and availability of products related to EpCAM antibody [E144] |
| |
EpCAM antibody [E144] - related products:
|
|
|
| |
EpCAM antibody [E144] - other tools:
|
Contact Abcam with a Technical Enquiry about EpCAM antibody [E144] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - EpCAM antibody [E144] (ab32392)](/index.html?datasheet=32392#ab32392_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - EpCAM antibody [E144] (ab32392)](/index.html?datasheet=32392#ab32392_2.jpg)
