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Anti-Epac2 antibody (ab21238)

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Overview

Product name

Anti-Epac2 antibody
See all Epac2 products (3) ...

Description

Rabbit polyclonal to Epac2

Specificity

This antibody does not cross react with other Epac family proteins.

Tested applications

WB, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Human

Immunogen

Synthetic peptide: CPREQFDSLTPLPEQEGPT, corresponding to amino acids 743-762 of Epac2

CPREQFDSLTPLPEQEGPT

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The affinity purified antibodies are isolated on immobilized antigen affinity column and supplied as stabilized product.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cGMP
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP

  • Western blot - Epac2 antibody (ab21238)Western blot - Epac2 antibody (ab21238) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab21238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.

Tissue specificity

Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.

Sequence similarities

Contains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.

Domain

The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP.

Cellular localization

Cytoplasm. Membrane.

Target information above from: UniProt accessionQ8WZA2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CAMP GEFII antibody
  • cAMP regulated guanine nucleotide exchange factor II antibody
  • cAMP-GEFII antibody
  • cAMP-regulated guanine nucleotide exchange factor II antibody
  • CAMPGEFII antibody
  • CGEF 2 antibody
  • CGEF2 antibody
  • Epac 2 antibody
  • EPAC2 antibody
  • Exchange factor directly activated by cAMP 2 antibody
  • Exchange protein directly activated by cAMP 2 antibody
  • Nbla00496 antibody
  • Putative protein product of Nbla00496 antibody
  • Rap guanine nucleotide exchange factor (GEF) 4 antibody
  • RAP guanine nucleotide exchange factor 4 antibody
  • RAPGEF 4 antibody
  • RAPGEF4 antibody
  • RPGF4_HUMAN antibody
see all

Database links

see all

Anti-Epac2 antibody images:

  Western blot - Epac2 antibody (ab21238)

Western blot - Epac2 antibody (ab21238)

Anti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg

Secondary
HRP-conjugated goat anti-rabbit IgG

Performed under reducing conditions.

Predicted band size : 112 kDa


Exposure time : 3 minutes

This image is courtesy of an Abreview submitted by Dr maria sabbatini

See Abreview

References for Anti-Epac2 antibody (ab21238)

This product has been referenced in:

  • Thomas M  et al. The Cellular DExD/H-Box RNA-Helicases UAP56 and URH49 Exhibit a CRM1-Independent Nucleocytoplasmic Shuttling Activity. PLoS One 6:e22671 (2011). ICC/IF; Human.Read more (PubMed: 21799930) »
  • Lau WK  et al. Differential Inhibitory Effects of CysLT(1) Receptor Antagonists on P2Y(6) Receptor-Mediated Signaling and Ion Transport in Human Bronchial Epithelia. PLoS One 6:e22363 (2011). ICC/IF; Human.Read more (PubMed: 21799837) »

See all 4 publications for this product

Publishing research using ab21238? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"