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Products:Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Overview
Product name
Anti-Eph receptor A5 antibody
See all Eph receptor A5 products (6) ...
Description
Goat polyclonal to Eph receptor A5
Specificity
Ab10612 recognizes recombinant mouse and rat EphA5 and a customer reported no signal in mouse brain lysate. Therefore it appears the antibody is not suitable for detection of endogenous EphA5. The antibody shows approximately 15% cross-reactivity with recombinant mouse EphA3, recombinant mouse EphA4, recombinant mouse EphA6, recombinant mouse EphB7, recombinant mouse EphA8, and recombinant rat EphB1.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Purified recombinant rat EphA5 extracellular domain expressed in mouse NSO cells.
General notes
Endotoxin level is < 0.15 EU (endotoxin units) per 1 µg antibody as determined by the LAL (Limulus amebocyte lysate) method.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity isolated antigen specific antibody is obtained from goat anti- EphA5 antiserum by immuno-specific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the peptide.
Primary antibody notes
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor family, which binds members of the Ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The extracellular domains of rat and mouse EphA5 share approximately 98.5% amino acid identity. Rat EphA5 shares 96.5% amino acid homology with human EphA5. The calculated molecular mass of the reduced rat EphA5/Fc monomer is approximately 84 kDa, but as a result of glycosylation, recombinant EphA5/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to kinase activation of the intrinsic kinase activity. EphA5 binds to Ephrin A1, Ephrin A2, Ephrin A3, Ephrin A4 and Ephrin A5. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat EphA5 is approximately 1.3 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant rat EphA5 is approximately 25 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 110 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor family, which binds members of the Ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The extracellular domains of rat and mouse EphA5 share approximately 98.5% amino acid identity. Rat EphA5 shares 96.5% amino acid homology with human EphA5. The calculated molecular mass of the reduced rat EphA5/Fc monomer is approximately 84 kDa, but as a result of glycosylation, recombinant EphA5/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to kinase activation of the intrinsic kinase activity. EphA5 binds to Ephrin A1, Ephrin A2, Ephrin A3, Ephrin A4 and Ephrin A5. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
Cellular localization
Type I membrane protein; almost exclusively expressed in the nervous system.
Alternative names
- bsk antibody
- CEK 7 antibody
- Eck like sequence 1 antibody
see all
Database links
- Entrez Gene: 13839 Mouse
- Entrez Gene: 79208 Rat
- SwissProt: Q60629 Mouse
- SwissProt: P54757 Rat
- Unigene: 4466 Mouse
References for Anti-Eph receptor A5 antibody (ab10612)
ab10612 has not yet been referenced specifically in any publications.
Publishing research using ab10612? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"