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Products:Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Overview
Product name
Anti-Eph receptor A5 antibody
See all Eph receptor A5 products (6) ...
Description
Rabbit polyclonal to Eph receptor A5
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 957-972 of Human Eph A5.
Positive control
Mouse brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 114 kDa (predicted molecular weight: 124 kDa).
IHC-P: 1/50 - 1/100.
Target
Relevance
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor family, which binds members of the Ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The extracellular domains of rat and mouse EphA5 share approximately 98.5% amino acid identity. Rat EphA5 shares 96.5% amino acid homology with human EphA5. The calculated molecular mass of the reduced rat EphA5/Fc monomer is approximately 84 kDa, but as a result of glycosylation, recombinant EphA5/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to kinase activation of the intrinsic kinase activity. EphA5 binds to Ephrin A1, Ephrin A2, Ephrin A3, Ephrin A4 and Ephrin A5. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
Cellular localization
Type I membrane protein; almost exclusively expressed in the nervous system.
Alternative names
- bsk antibody
- CEK 7 antibody
- Eck like sequence 1 antibody
see all
Database links
- Entrez Gene: 2044 Human
- Entrez Gene: 13839 Mouse
- Omim: 600004 Human
- SwissProt: P54756 Human
- SwissProt: Q60629 Mouse
- Unigene: 4466 Mouse
Anti-Eph receptor A5 antibody images:
Western blot - Eph receptor A5 antibody (ab5398)
Predicted band size : 124 kDa
ab5398 at a 1/100 dilution staining approximately 114kDa band of Eph A5 in mouse brain tissue by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor A5 antibody (ab5398)
ab5398 staining Eph receptor A5 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-Eph receptor A5 antibody (ab5398)
ab5398 has not yet been referenced specifically in any publications.
Publishing research using ab5398? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"