Anti-Eph receptor B4/HTK antibody (ab64820)
Key features and details
- Rabbit polyclonal to Eph receptor B4/HTK
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor B4/HTK antibody
See all Eph receptor B4/HTK primary antibodies -
Description
Rabbit polyclonal to Eph receptor B4/HTK -
Host species
Rabbit -
Specificity
ab64820 detects endogenous levels of total Eph receptor B4 protein. We have not performed cross-reactivity testing to other class B Eph receptors. The immunogen has high homology to EPHB1(100%), EPHB3 (100%), EPHB2 (92%). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Eph receptor B4/HTK aa 550-650 (internal sequence).
Database link: P54760 -
Positive control
- WB: 293 cell, Jurkat, HY926 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab64820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
Target
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Function
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
Tissue specificity
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2050 Human
- Omim: 600011 Human
- SwissProt: P54760 Human
- Unigene: 437008 Human
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Alternative names
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
Images
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All lanes : Anti-Eph receptor B4/HTK antibody (ab64820) at 1/500 dilution
Lane 1 : 293 cell extract at 30 µg
Lane 2 : Jurkat cell extract at 30 µg
Lane 3 : immunising peptide at 10 µg
Predicted band size: 108 kDa
Observed band size: 108 kDa -
IHC image of ab64820 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64820, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Anti-Eph receptor B4/HTK antibody (ab64820) at 1/2000 dilution + HY926 cell lysate
Predicted band size: 108 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab64820 has been referenced in 5 publications.
- Miyagawa K et al. Osteoclast-derived IGF1 is required for pagetic lesion formation in vivo. JCI Insight 5:N/A (2020). PubMed: 32078587
- Fehnel KP et al. Dysregulation of the EphrinB2-EphB4 ratio in pediatric cerebral arteriovenous malformations is associated with endothelial cell dysfunction in vitro and functions as a novel noninvasive biomarker in patients. Exp Mol Med 52:658-671 (2020). PubMed: 32286515
- Protack CD et al. Eph-B4 regulates adaptive venous remodeling to improve arteriovenous fistula patency. Sci Rep 7:15386 (2017). PubMed: 29133876
- Model LS et al. Arterial shear stress reduces eph-b4 expression in adult human veins. Yale J Biol Med 87:359-71 (2014). IHC-P ; Human . PubMed: 25191151
- Doleman JF et al. Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29). Nutr Metab (Lond) 7:56 (2010). WB ; Human . PubMed: 20624275