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Anti-Eph receptor B2 antibody (ab54650)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
    • Tissue specificityBrain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
    • Involvement in diseaseDefects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Cellular localizationMembrane.

      • Target information above from: UniProt accession P29323 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        cek5 antibodyDevelopmentally regulated EPH related tyrosine kinase antibodyDRT antibody
        EK5 antibodyELK related protein tyrosine kinase antibodyEph receptor B2 antibodyEPH tyrosine kinase 3 antibodyEPH-like kinase 5 antibodyEphB2 antibodyEPHB2_HUMAN antibodyEphrin type B receptor 2 antibodyEphrin type-B receptor 2 antibodyEPHT 3 antibodyERK antibodyETECK antibodyhEK5 antibodyNuk antibodyPrkm 5 antibodyReceptor protein tyrosine kinase HEK 5 antibodyRenal carcinoma antigen NY-REN-47 antibodySek 3 antibodyTyro 5 antibodyTyrosine protein kinase receptor CEK 5 antibodyTyrosine protein kinase receptor EPH 3 antibodyTyrosine protein kinase receptor QEK 5 antibodyTyrosine-protein kinase receptor EPH-3 antibodyTyrosine-protein kinase TYRO5 antibody
      see all

    Anti-Eph receptor B2 antibody images

    • Western blot - Eph receptor B2 antibody (ab54650)
      Western blot - Eph receptor B2 antibody (ab54650)


      Predicted band size : 118 kDa
      Eph receptor B2 antibody (ab54650) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

    References for Anti-Eph receptor B2 antibody (ab54650)

    ab54650 has not yet been referenced specifically in any publications.

    Publishing research using ab54650 ? Please let us know so that we can cite the reference in this datasheet

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Eph receptor B2 antibody

     Inconclusive
    Abreviews
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse lung containing artery)
    Specification mouse lung containing artery
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: dako low pH
    Permeabilization No
    Blocking step HP as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
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    Submitted Nov 10 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"