Overview
Product nameEph receptor B2 protein (Active)See all Eph receptor B2 proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 569-1055 of Human Eph receptor B2 with N terminal His tag.
Expression hostBaculovirus infected sf9 cells
Properties
Purification notes>80% purity by SDS-PAGE
Biological activityActivity: 150 U/mg. One unit is defined as the amount of enzyme that will
phosphorylate 1 pmol of tyrosine substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM
HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP,
30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant EPHB2.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Concentration information loading...
Additional notesExpressed in a Baculovirus infected Sf9 expression system. Expected molecular weight of 52 kDa.
Sequence notesF ERADSEYTDK LQHYTSGHMT PGMKIYIDPF TYEDPNEAVR EFAKEIDISC VKIEQVIGAG EFGEVCSGHL KLPGKREIFV AIKTLKSGYT EKQRRDFLSE ASIMGQFDHP NVIHLEGVVT KSTPVMIITE FMENGSLDSF LRQNDGQFTV IQLVGMLRGI AAGMKYLADM NYVHRDLAAR NILVNSNLVC KVSDFGLSRF LEDDTSDPTY TSALGGKIPI RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM TNQDVINAIE QDYRLPPPMD CPSALHQLML DCWQKDRNHR PKFGQIVNTL DKMIRNPNSL KAMAPLSSGI NLPLLDRTIP DYTSFNTVDE WLEAIKMGQY KESFANAGFT SFDVVSQMMM EDILRVGVTL AGHQKKILNS IQVMRAQMNQ IQSVEGQPLA RRPRATGRTK RCQPRDVTKK TCNSNDGKKK GMGKKKTDPG RGREIQGIFF KEDSHKESND CSCGG
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab42607
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| Inhibition Assay |
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Application notesInhib: Use at an assay dependent dilution.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
cek5Developmentally regulated EPH related tyrosine kinaseDRT
EK5ELK related protein tyrosine kinaseEph receptor B2EPH tyrosine kinase 3EPH-like kinase 5EPHB2EPHB2_HUMANEphrin type B receptor 2Ephrin type-B receptor 2EPHT 3ERKETECKhEK5NukPrkm 5Receptor protein tyrosine kinase HEK 5Renal carcinoma antigen NY-REN-47Sek 3Tyro 5Tyrosine protein kinase receptor CEK 5Tyrosine protein kinase receptor EPH 3Tyrosine protein kinase receptor QEK 5Tyrosine-protein kinase receptor EPH-3Tyrosine-protein kinase TYRO5
see all
FunctionReceptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
Tissue specificityBrain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
Involvement in diseaseDefects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationMembrane.
References for Eph receptor B2 protein (Active) (ab42607)
ab42607
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
