Eph receptor B4 antibody [4A12G8,5G2F8] (ab66336)

Product Name 

Eph receptor B4 antibody [4A12G8,5G2F8]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4A12G8,5G2F8] to Eph receptor B4

Immunogen 

Recombinant His-tagged fragment expressed in E.Coli strain BL21 (DE3), corresponding to amino acids 771-987 of Human Eph receptor B4

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000. Predicted molecular weight: 108 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Lung cancer.

Cellular localization 

Cell Membrane

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors

Relevance 

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which bind the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4A12G8,5G2F8

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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