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Anti-Eph receptor B4 antibody [7H4A6] (ab70404)

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Overview

Product name

Anti-Eph receptor B4 antibody [7H4A6]
See all Eph receptor B4 products (8) ...

Description

Mouse monoclonal [7H4A6] to Eph receptor B4

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Horse, Cow, Dog, Chimpanzee

Immunogen

Purified Recombinant tagged fragment: CLRKQSNGRE AEYSDKHGQY LIGHGTKVYI DPFTYEDPNE AVREFAKEID V, corresponding to amino acids 562-612 of Human Eph receptor B4 expressed in E.coli strain BL21 (DE3)

CLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNE AVREFAKEIDV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Truncated tagged Eph receptor B4 recombinant protein.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

7H4A6

Isotype

IgG1

Research areas

Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors

  • Western blot - Eph receptor B4 antibody [7H4A6] (ab70404)Western blot - Eph receptor B4 antibody [7H4A6] (ab70404) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/10000.
WB: 1/500 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 108 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.

Tissue specificity

Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.

Post-translational
modifications

Autophosphorylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP54760 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EPHB4 antibody
  • EPHB4_HUMAN antibody
  • Ephrin type-B receptor 4 antibody
  • Hepatoma Transmembrane Kinase antibody
  • HTK antibody
  • MYK1 antibody
  • TYRO11 antibody
  • Tyrosine-protein kinase receptor HTK antibody
  • Tyrosine-protein kinase TYRO11 antibody
see all

Database links

Anti-Eph receptor B4 antibody [7H4A6] images:

  Western blot - Eph receptor B4 antibody [7H4A6] (ab70404)

Western blot - Eph receptor B4 antibody [7H4A6] (ab70404)

All lanes : Anti-Eph receptor B4 antibody [7H4A6] (ab70404) at 1/2000 dilution

Lane 1 : Cell lysates prepared from Jurkat cell
Lane 2 : Cell lysates prepared from HEK293 cells

Lysates/proteins at 100 µg per lane.

Secondary
HRP-conjugated Goat polyclonal to mouse IgG1

Predicted band size : 108 kDa

References for Anti-Eph receptor B4 antibody [7H4A6] (ab70404)

This product has been referenced in:

  • Doleman JF  et al. Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29). Nutr Metab (Lond) 7:56 (2010). WB; Human.Read more (PubMed: 20624275) »

See 1 publication for this product

Publishing research using ab70404? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"