![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259) image](#Eph-receptor-B4-Primary-antibodies-ab73259-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259) image](#Eph-receptor-B4-Primary-antibodies-ab73259-5.jpg)
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Products:Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Overview
Product name
Anti-Eph receptor B4 antibody [RM0056-6A14]
See all Eph receptor B4 products (8) ...
Description
Rat monoclonal [RM0056-6A14] to Eph receptor B4
Specificity
ab73259 is specific for mouse Eph receptor B4. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant mouse Eph receptor B4 extracellular domain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
ab73259 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse Eph receptor B4 extracellular domain).
Clonality
Monoclonal
Clone number
RM0056-6A14
Isotype
IgG1
Research areas
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/1000.Predicted molecular weight: 109 kDa.
IHC-P: 1/100 - 1/200.
Target
Function
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
Tissue specificity
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP54760
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EPHB4 antibody
- EPHB4_HUMAN antibody
- Ephrin type-B receptor 4 antibody
see all
Database links
- Entrez Gene: 13846 Mouse
- SwissProt: P54761 Mouse
- Unigene: 34533 Mouse
Anti-Eph receptor B4 antibody [RM0056-6A14] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259)](/ps/datasheet/images/73/ab73259/Eph-receptor-B4-Primary-antibodies-ab73259-1.jpg)
ab73259 at 1/200 dilution, staining Eph receptor B4 in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B4 antibody [RM0056-6A14] (ab73259)](/ps/datasheet/images/73/ab73259/Eph-receptor-B4-Primary-antibodies-ab73259-5.jpg)
ab73259 staining the Eph receptor B4 in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Eph receptor B4 antibody [RM0056-6A14] (ab73259)
ab73259 has not yet been referenced specifically in any publications.
Publishing research using ab73259? Please let us know so that we can cite the reference in this datasheet
![Eph receptor B4 antibody [RM0056-6A14] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (73259)](/ps/datasheet/images/73/ab73259/Eph-receptor-B4-Primary-antibodies-ab73259-4.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"