Eph receptor B4 antibody [RM0056-6A14] (ab73259)

Product Name 

Eph receptor B4 antibody [RM0056-6A14]

Product type 

Primary antibodies

Description 

Rat monoclonal [RM0056-6A14] to Eph receptor B4

Immunogen 

Recombinant mouse Eph receptor B4 extracellular domain

Reacts with 
(species key)

Ms

Specificity 

ab73259 is specific for mouse Eph receptor B4. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/200.
WB: 1/100 - 1/1000. Predicted molecular weight: 109 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors

Relevance 

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which bind the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

RM0056-6A14

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS

Purification notes 

ab73259 was produced from a hybridoma (mouse myeloma fused with
spleen cells from a rat immunized with recombinant mouse Eph receptor B4 extracellular domain).

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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