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Product Name
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Eph receptor B4 antibody
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See all Eph receptor B4 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Eph receptor B4
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Immunogen
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Purified recombinant mouse EphB4 extracellular domain expressed in mouse NSO cells.
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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Dot, ELISA, Inhib
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Abreviews
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Application notes
(see key)
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Recommended dilutions Dot: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EphB4 is approximately 5 ng/lane under non-reducing and reducing conditions. ELISA: Use at a concentration of 0.5 - 1.0 µg/ml, detection limit 1.5 ng/well. Inhib: Use at a concentration of 0.5 - 2 µg/ml. For neutralization of receptor-ligand interaction, approximately 0.5-2 µg/ml of the antibody will block 50% of the binding of recombinant mouse ephrin-B2/ (5 ng/ml) to immobilized recombinant mouse EphB4/Fc chimera (100 µl of a 2 µg/ml solution coated in each well).
The recombinant mouse EphB4 used for the testing of this antibody consists of the extracellular domain of mouse EphB4 (amino acids 1-539) fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB4/Fc has Leu 16 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.2 kDa, but as a result of glycosylation, recombinant EphB4/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. EphB4 binds to ephrin-B1 and ephrin-B2. Human and mouse EphB4 extracellular domains share approximately 88% homology.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Surface Molecules Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
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Relevance
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EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which bind the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 13846 (Mouse)
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None Constituents: PBS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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