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Anti-Eph receptor B4 antibody (ab10618)

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Overview

Product name

Anti-Eph receptor B4 antibody
See all Eph receptor B4 products (8) ...

Description

Goat polyclonal to Eph receptor B4

Tested applications

ELISA, Dot Blot, Inhibition Assaymore details

Cross reactivity

Reacts with

Mouse

Immunogen

Purified recombinant mouse EphB4 extracellular domain expressed in mouse NSO cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     
  • Dot Blot

     
  • Inhibition Assay

     

Application notes

Dot: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EphB4 is approximately 5 ng/lane under non-reducing and reducing conditions.
ELISA: Use at a concentration of 0.5 - 1.0 µg/ml, detection limit 1.5 ng/well.
Inhib: Use at a concentration of 0.5 - 2 µg/ml. For neutralization of receptor-ligand interaction, approximately 0.5-2 µg/ml of the antibody will block 50% of the binding of recombinant mouse ephrin-B2/ (5 ng/ml) to immobilized recombinant mouse EphB4/Fc chimera (100 µl of a 2 µg/ml solution coated in each well).

The recombinant mouse EphB4 used for the testing of this antibody consists of the extracellular domain of mouse EphB4 (amino acids 1-539) fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB4/Fc has Leu 16 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.2 kDa, but as a result of glycosylation, recombinant EphB4/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. EphB4 binds to ephrin-B1 and ephrin-B2. Human and mouse EphB4 extracellular domains share approximately 88% homology.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.

Tissue specificity

Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.

Post-translational
modifications

Autophosphorylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP54760 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Ephb4 antibody
  • EPHB4_HUMAN antibody
  • Ephrin type-B receptor 4 antibody
  • Hepatoma Transmembrane Kinase antibody
  • HTK antibody
  • MYK1 antibody
  • TYRO11 antibody
  • Tyrosine-protein kinase receptor HTK antibody
  • Tyrosine-protein kinase TYRO11 antibody
see all

References for Anti-Eph receptor B4 antibody (ab10618)

ab10618 has not yet been referenced specifically in any publications.

Publishing research using ab10618? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"