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Products:Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Overview
Product name
Anti-Eph receptor B4 antibody
See all Eph receptor B4 products (8) ...
Description
Rabbit polyclonal to Eph receptor B4
Specificity
ab64820 detects endogenous levels of total Eph receptor B4 protein.
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from internal region of human Eph receptor B4.
Positive control
293 and Jurkat cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab64820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cardiovascular >> Angiogenesis >> Ephrins >> Eph Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Target
Function
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
Tissue specificity
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP54760
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EPHB4 antibody
- EPHB4_HUMAN antibody
- Ephrin type-B receptor 4 antibody
see all
Database links
- Entrez Gene: 2050 Human
- Entrez Gene: 13846 Mouse
- Omim: 600011 Human
- SwissProt: P54760 Human
- SwissProt: P54761 Mouse
- Unigene: 437008 Human
- Unigene: 34533 Mouse
Anti-Eph receptor B4 antibody images:
Western blot - Eph receptor B4 antibody (ab64820)
All lanes : Anti-Eph receptor B4 antibody (ab64820) at 1/500 dilution
Lane 1 : 293 cell extract at 30 µg
Lane 2 : Jurkat cell extract at 30 µg
Lane 3 : immunising peptide at 10 µg
Predicted band size : 108 kDa
Observed band size : 108 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Eph receptor B4 antibody(ab64820)
IHC image of ab64820 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64820, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Eph receptor B4 antibody (ab64820)
This product has been referenced in:
- Doleman JFet al. Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29). Nutr Metab (Lond) 7:56 (2010). WB; Human.Read more (PubMed: 20624275) »
See 1 publication for this product
Publishing research using ab64820? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"