![Western blot - Eph receptor B6 antibody [2A6B9] (ab66325) image](#eph61.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B6 antibody [2A6B9] (ab66325) image](#ab66325-3.jpg)
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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-Eph receptor B6 antibody [2A6B9]
See all Eph receptor B6 products (4) ...
Description
Mouse monoclonal [2A6B9] to Eph receptor B6
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 601-750 of Human Eph receptor B6, expressed in E.Coli strain BL21 (DE3) and containing an N-terminal 6 His tag.
Positive control
Human lymph node tissue Human brain tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
2A6B9
Isotype
IgG1
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000.Predicted molecular weight: 109 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/500 - 1/2000.
Target
Function
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Tissue specificity
Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Domain
The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2.
Post-translational
modifications
Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion.
Cellular localization
Secreted and Membrane.
Target information above from: UniProt accessionO15197
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EPH receptor B6 antibody
- EphB6 antibody
- EPHB6_HUMAN antibody
see all
Database links
- Entrez Gene: 2051 Human
- Omim: 602757 Human
- SwissProt: O15197 Human
- Unigene: 380089 Human
Anti-Eph receptor B6 antibody [2A6B9] images:
Western blot - Eph receptor B6 antibody [2A6B9] (ab66325)
![Western blot - Eph receptor B6 antibody [2A6B9] (ab66325)](/ps/datasheet/Images/66/ab66325/eph61.jpg)
Anti-Eph receptor B6 antibody [2A6B9] (ab66325) at 1/500 dilution + truncated Eph receptor B6 recombinant protein at 1 µg
Predicted band size : 109 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
The observed band size does not correspond to the predicted protein size as a recombinant fragment was used in the test lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B6 antibody [2A6B9] (ab66325)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B6 antibody [2A6B9] (ab66325)](/ps/datasheet/Images/66/ab66325/ab66325-3.jpg)
Immunohistochemical analysis of paraffin embedded human lymph node and brain showing cytoplasmic localisation with DAB staining using ab66325 at 1/500 dilution.
References for Anti-Eph receptor B6 antibody [2A6B9] (ab66325)
ab66325 has not yet been referenced specifically in any publications.
Publishing research using ab66325? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"