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Anti-Eph receptor B6 antibody (ab54656)

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Overview

Product name

Anti-Eph receptor B6 antibody
See all Eph receptor B6 products (4) ...

Description

Mouse monoclonal to Eph receptor B6

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant fragment: DTTGETSEIG WLTYPPGGWD EVSVLDDQRR LTRTFEACHV AGAPPGTGQD NWLQTHFVER RGAQRAHIRL HFSVRACSSL GVSGGTCRET FTLYYRQAEE , corresponding to amino acids 23-122 of Human Eph receptor B6

DTTGETSEIG WLTYPPGGWD EVSVLDDQRR LTRTFEACHV AGAPPGTGQD NWLQTHFVER RGAQRAHIRL HFSVRACSSL GVSGGTCRET FTLYYRQAEE

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Research areas

Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

  • IHC-P - Eph receptor B6 antibody (ab54656)IHC-P - Eph receptor B6 antibody (ab54656) image (enlarge)

  • Western blot - Eph receptor B6 antibody (ab54656)Western blot - Eph receptor B6 antibody (ab54656) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.

Tissue specificity

Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.

Domain

The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2.

Post-translational
modifications

Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion.

Cellular localization

Secreted and Membrane.

Target information above from: UniProt accessionO15197 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EPH receptor B6 antibody
  • Ephb6 antibody
  • EPHB6_HUMAN antibody
  • EPHRIN RECEPTOR EphB6 antibody
  • Ephrin type B receptor 6 precursor antibody
  • Ephrin type-B receptor 6 antibody
  • HEP antibody
  • MGC129910 antibody
  • MGC129911 antibody
  • Tyrosine protein kinase defective receptor EPH 6 antibody
  • Tyrosine-protein kinase-defective receptor EPH-6 antibody
see all

Database links

see all

Anti-Eph receptor B6 antibody images:

  IHC-P - Eph receptor B6 antibody (ab54656)

IHC-P - Eph receptor B6 antibody (ab54656)

Eph receptor B6 antibody (ab54656) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.

  Western blot - Eph receptor B6 antibody (ab54656)

Western blot - Eph receptor B6 antibody (ab54656)

Eph receptor B6 antibody (ab54656) at 1ug/lane + PC-12 cell lysate at 25ug/lane.

References for Anti-Eph receptor B6 antibody (ab54656)

ab54656 has not yet been referenced specifically in any publications.

Publishing research using ab54656? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"