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All lanes : Anti-Eph receptor B6 antibody (ab70181) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells
Lane 4 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.
HeLa cells with ab70181 at 1/500 dilution, with and without the immunising peptide.
Human brain tissue with ab70181 at 1/50 dilution, with and without the immunising peptide.
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