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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-Eph receptor B6 antibody
See all Eph receptor B6 products (4) ...
Description
Rabbit polyclonal to Eph receptor B6
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal region of human Eph receptor B6.
Positive control
HeLa cells, human brain tissue, extracts from HeLa, 293 and COLO cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Function
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Tissue specificity
Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Domain
The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2.
Post-translational
modifications
Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion.
Cellular localization
Secreted and Membrane.
Target information above from: UniProt accessionO15197
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EPH receptor B6 antibody
- EphB6 antibody
- EPHB6_HUMAN antibody
see all
Database links
- Entrez Gene: 2051 Human
- Entrez Gene: 13848 Mouse
- Entrez Gene: 312275 Rat
- Omim: 602757 Human
- SwissProt: O15197 Human
- SwissProt: O08644 Mouse
- SwissProt: P0C0K7 Rat
- Unigene: 380089 Human
see all
Anti-Eph receptor B6 antibody images:
Western blot - Eph receptor B6 antibody (ab70181)
All lanes : Anti-Eph receptor B6 antibody (ab70181) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells
Lane 4 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Eph receptor B6 antibody (ab70181)
HeLa cells with ab70181 at 1/500 dilution, with and without the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Eph receptor B6 antibody (ab70181)
Human brain tissue with ab70181 at 1/50 dilution, with and without the immunising peptide.
References for Anti-Eph receptor B6 antibody (ab70181)
ab70181 has not yet been referenced specifically in any publications.
Publishing research using ab70181? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"