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Ephrin B2 protein (Fc Chimera His Tag) (ab92862)

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Overview

  • Product nameEphrin B2 protein (Fc Chimera His Tag)See all Ephrin B2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-227 of Human Ephrin B2 fused with Fc and a 6xHis tag, expressed in HEK293E cells; 472 aa with tag, MWt 52.9kDa (UniProt ID = P52799).
  • Expression hostHEK 293 cells

    • Properties

  • Purification notesPurified by affinity chromatography.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Additional notesAfter reconstitution, store in working aliquots at -80ºC.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92862 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    ELISA
    SDS-PAGE
  • Application notesELISA: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        EFN B2EFNB 2EFNB2
        EFNB2_HUMANEph related receptor tyrosine kinase ligand 5EPH-related receptor tyrosine kinase ligand 5ephrin B2Ephrin-B2EphrinB2EPLG 5EPLG5Htk LHTK ligandHTK-LHTKLLERK 5LERK-5LERK5Ligand of eph related kinase 5MGC126226MGC126227MGC126228OTTMUSP00000024973
      see all
  • FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
  • Tissue specificityLung and kidney.
  • Sequence similaritiesBelongs to the ephrin family.
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.
  • Post-translational
    modifications    Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P52799 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Ephrin B2 protein (Fc Chimera His Tag) images

    • SDS-PAGE - Ephrin B2 protein (Fc Chimera His Tag) (ab92862)
      SDS-PAGE - Ephrin B2 protein (Fc Chimera His Tag) (ab92862)
      10% SDS-PAGE of ab92862 stained with Coomassie Blue (CB) and immunoblotted with polyclonal antibody against Human Ephrin B2 (WB)

    References for Ephrin B2 protein (Fc Chimera His Tag) (ab92862)

    ab92862 has not yet been referenced specifically in any publications.

    Publishing research using ab92862 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab92862 Submit a Question for ab92862

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    Can I know the purity (of estimated purity) of the product?

    Purity: ³95% (SDS-PAGE)

    Expressed in HEK293 cells

    I hope this is helpful. Please contact me again if you have any further questions.

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    I refer to the product Ephrin B2 protein (Fc Chimera His Tag) (ab92862).
    It is mentioned that the concentration for the product is 1 mg/ml, is this the concentration for the
    fusion protein? If so, a conversion of the concentration in terms ...

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    Thank you for your enquiry.

    Yes, it is the fusion protein (472 aa with tag, MWt 52.9kDa) that is at 1mg/ml concentration. The mass of the fusion protein includes the 6x His tag.

    I hope this is helpful. Please contact me again if you ha...

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    1) wo ist der his tag? nicht deutlich auf DS 2) Koennen Sie mir alle "Fc chimera mit his-tag" Produkte nennen? vorzugsweise Rezeptorproteine

    Vielen Dank für Ihre Geduld! Endlich (!) hat das Labor, welches ab92862 herstellt, sich mit der Information zur Konstruktion des Ephrin B2-Fc-Chimärproteins gemeldet. Es besteht vom N- zum C-Terminus aus: EphrinB2 -IgG1Fc -Histag (Details siehe Anhan...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"