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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
EpiSeeker Bisulfite Modification Kit - Whole Cell (ab117126) allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. ab117126 is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.
Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
NOTE: If you use ab117126 for MSP, the numbers of PCR cycles should be greater than 45.
The kit can be stored at room temperature (20-22°C) away from light (with the exception of component EBM1 (Digestion Powder) for 6 months from the shipping date.
EBM1 should be stored at –20°C, or stored at 4°C as soon as it is dis-solved in EBM2 (up to 6 months).
Each vial of EBM4 can be used for 10 sample treatments.
The prepared EBM4/EBM5/EBM6 solution should be used immediately, unless it is stored at –20°C away from light (up to one week).
Frozen EBM4/EBM5/EBM6 solution must be thawed at room temperature and vortexed for 2 minutes prior to use.
|Components||40 tests||80 tests|
|EBM1 (Digestion Powder)||1 vial||1 vial|
|EBM2 (Digestion Solution)||1 x 0.1ml||1 x 0.2ml|
|EBM3 (Cell Collection Buffer)||1 x 1ml||1 x 2ml|
|EBM4 (DNA Modification Powder)||4 vials||8 vials|
|EBM5 (DNA Modification Buffer)||1 x 5ml||1 x 10ml|
|EBM6 (Balance Buffer)||1 x 0.4ml||1 x 0.8ml|
|EBM7 (DNA Binding Buffer)||1 x 14ml||1 x 28ml|
|EBM8 (Modified DNA Elution)||1 x 1ml||1 x 2ml|
|F-Collection Tube||1 x 40 units||1 x 80 units|
|F-Spin Column||1 x 40 units||1 x 80 units|
Our Abpromise guarantee covers the use of ab117126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. Minimum starting material: 100-20000 cells / 1-100µg tissue / 0.2-2mm2 tissue section sample. Optimal starting material: 500-5000 cells / 5-20µg tissue / 0.5-1mm2 tissue section.|
ab117126 has not yet been referenced specifically in any publications.
Publishing research using ab117126 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"