EpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue (ab117150)

Overview

  • Product nameEpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue
    See all Histone H3 (acetylation) kits
  • Sample type
    Tissue
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

    EpiSeeker ChIP Kit - Histone H3 (acetyl)-Tissue (ab117150) enables the user to investigate in vivo H3 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

    If you are using cells (adherent or in suspension) as starting material, we recommend the use of a EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148)

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H3, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsChIPmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    100X Protease Inhibitor Cocktail 1 x 25µl 1 x 40µl
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-Acetyl Histone H3 (1 mg/ml) 1 x 25µl 1 x 50µl
    ECA1 (Wash Buffer) 1 x 28ml 2 x 28ml
    ECA2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    ECA3 (Lysis Buffer) 1 x 4ml 1 x 6ml
    ECA4 (ChIP Dilution Buffer) 1 x 4ml 1 x 6ml
    ECA5 (DNA Release Buffer) 1 x 1ml 2 x 2ml
    ECA6 (Reverse Buffer) 1 x 1ml 2 x 2ml
    ECA7 (Binding Buffer) 1 x 5ml 1 x 8ml
    ECA8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Homogenizing Buffer 1 x 5ml 1 x 10ml
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117150 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ChIP Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue (ab117150)

    ab117150 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.


    In our testing, the promoter region of Ngal gene in MCF-7 cells is enriched by acetyl H3and could be used as a positive control.



    I hope this information helps. Please contact us with any ...

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    I am sorry for the delay in our reply. Here are answers to your questions: 1. The antibodies included in ab117136, 117144, 117145, 117147, 117150 and117151 are not sold separately in our catalog. 2. The antibodies in kits ab117144, 117145 and117147 hav...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"