EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148)

Overview

  • Product nameEpiSeeker ChIP Kit - Histone H3 (acetyl)See all Histone H3 (acetylation) kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human, all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

    EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148) enables the user to investigate in vivo H3 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

    If you are using tissue as your starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue (ab117150)

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H3, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsChIPmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-Acetyl-Histone H3 (1 mg/ml) 1 x 25µl 1 x 50µl
    ECA1 (Wash Buffer) 1 x 28ml 2 x 28ml
    ECA2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    ECA3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml
    ECA3B (Lysis Buffer) 1 x 2ml 2 x 2ml
    ECA4 (ChIP Dilution Buffer) 1 x 2ml 2 x 2ml
    ECA5 (DNA Release Buffer) 1 x 1ml 1 x 2ml
    ECA6 (Reverse Buffer) 1 x 1ml 1 x 2ml
    ECA7 (Binding Buffer) 1 x 5ml 1 x 8ml
    ECA8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Protease Inhibitor Cocktail (100X) 1 x 25µl 1 x 40µl
    Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117148 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ChIP Use at an assay dependent concentration.

    EpiSeeker ChIP Kit - Histone H3 (acetyl) images

    • Chromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECA4) and 2mg of ab117148 (blue). 2µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

    Protocols

    References for EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148)

    ab117148 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.


    Your homogenizing buffer could be used if the compositions of the bufferare similar to that of cytoplasmiclysis bufferand donotbreak nuclei to release chromatin.The buffer ECA3A may be used for tissues with h...

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    This kit recognizes acetyl H3K9/14.

    Thank you for contacting

    FOr your ChIP experiment 1 ul of elute could be sufficient. In general, the concentration of DNA is about 3-12 ng/ul (if elute is 20 ul) depending on the starting materials.

    I hope this information is helpful...

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    Thank you for your patience.

    The lab sent me the following explanation:

    The kit is designed for 24 reactions (not 24 samples). Thus, 25 ul of proteinase K should be sufficient for 24 reactionsas only 1 ul of proteinase K is require...

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    Thank you for getting back to me.

    I can understand your reservation in wanting to try the experiment again. I don't think leaving the DNA sample to stand with the column for a minute will in itself improve the results much but I think that th...

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    Thank you for providing that information.

    I have discussed this case with the source of this kit. Based on the information provided it seems that the problem is either with the way that the DNA is bound to the column, or with how it is eluted...

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    The kit developer says that the ECA7 buffer does not contain chaotropic reagents. Let me know if you continue to have difficulty isolating the DNA.

    Thank you for providing that information.

    I am sorry to have to ask you those questions as it is clear you have followed the protocol as it is written but we often find that even when the protocols to kits are used, it is often quite easy to ...

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    In ...

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    Thank you for your call last week and for your patience while I have been in touch with the lab. I apologize for the delay getting back to you.

    The easiest way to store all of your samples so that you can run them together would be to freeze ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"