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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.
EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148) enables the user to investigate in vivo H3 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
If you are using tissue as your starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue (ab117150)
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H3, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||24 tests||48 tests|
|100X Protease Inhibitor Cocktail||1 x 25µl||1 x 40µl|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-Acetyl-Histone H3 (1 mg/ml)||1 x 25µl||1 x 50µl|
|ECA1 (Wash Buffer)||1 x 28ml||2 x 28ml|
|ECA2 (Antibody Buffer)||1 x 15ml||1 x 30ml|
|ECA3A (Pre-Lysis Buffer)||1 x 4ml||1 x 8ml|
|ECA3B (Lysis Buffer)||1 x 2ml||2 x 2ml|
|ECA4 (ChIP Dilution Buffer)||1 x 2ml||2 x 2ml|
|ECA5 (DNA Release Buffer)||1 x 1ml||1 x 2ml|
|ECA6 (Reverse Buffer)||1 x 1ml||1 x 2ml|
|ECA7 (Binding Buffer)||1 x 5ml||1 x 8ml|
|ECA8 (Elution Buffer)||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Normal Mouse IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
Our Abpromise guarantee covers the use of ab117148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
ab117148 has not yet been referenced specifically in any publications.
Publishing research using ab117148 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"