Overview
- Product nameEpiSeeker ChIP Kit - Histone H3 (acetyl)See all Histone H3 (acetylation) kits ...
- Sample typeAdherent cells, Suspension cells
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human, all Mammals
- Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.
EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148) enables the user to investigate in vivo H3 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
If you are using tissue as your starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (acetyl) - Tissue (ab117150)
- Notes
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H3, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsChIP more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-Acetyl-Histone H3 (1 mg/ml) 1 x 25µl 1 x 50µl ECA1 (Wash Buffer) 1 x 28ml 2 x 28ml ECA2 (Antibody Buffer) 1 x 15ml 1 x 30ml ECA3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml ECA3B (Lysis Buffer) 1 x 2ml 2 x 2ml ECA4 (ChIP Dilution Buffer) 1 x 2ml 2 x 2ml ECA5 (DNA Release Buffer) 1 x 1ml 1 x 2ml ECA6 (Reverse Buffer) 1 x 1ml 1 x 2ml ECA7 (Binding Buffer) 1 x 5ml 1 x 8ml ECA8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl Protease Inhibitor Cocktail (100X) 1 x 25µl 1 x 40µl Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ChIP | ChIP |
EpiSeeker ChIP Kit - Histone H3 (acetyl) images
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ChIPChromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECA4) and 2mg of ab117148 (blue). 2µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Protocols
References for EpiSeeker ChIP Kit - Histone H3 (acetyl) (ab117148)
ab117148 has not yet been referenced specifically in any publications.
