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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
The position of the histone H3 at lysine 27 H3 (K27)) is important for epigenetic regulation of genes, as it can be either methylated or acetylated. It has been demonstrated that switching H3 (acetyl K27) to methylation on H3 (methyl K27) leads to chromatin gene silencing in many organisms. Thus, identification of genes silenced through H3 (methyl K27) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.
EpiSeeker ChIP Kit - Histone H3 (methyl K27)-Tissue (ab117147) enables the user to investigate in vivo H3 (methyl K27)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
Ab117147 uses an antibody that detects Histone H3 di-methylated on K27.
If you are using cells (adherent or in suspension) as starting material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (methyl K27) (ab117146)
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Dimethyl-H3-K27, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||24 tests||48 tests|
|1000X Protease Inhibitor Cocktail||1 x 25µl||1 x 40µl|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-Dimethyl-H3-K27 (1 mg/ml)||1 x 25µl||1 x 50µl|
|ECM1 (Wash Buffer)||1 x 28ml||2 x 28ml|
|ECM2 (Antibody Buffer)||1 x 15ml||1 x 30ml|
|ECM3 (Lysis Buffer)||1 x 4ml||1 x 6ml|
|ECM4 (ChIP Dilution Buffer)||1 x 4ml||1 x 6ml|
|ECM5 (DNA Release Buffer)||1 x 1ml||1 x 2ml|
|ECM6 (Reverse Buffer)||1 x 1ml||1 x 2ml|
|ECM7 (Binding Buffer)||1 x 5ml||1 x 8ml|
|ECM8 (Elution Buffer)||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Homogenizing Buffer||1 x 5ml||1 x 10ml|
|Normal Mouse IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
Our Abpromise guarantee covers the use of ab117147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
ab117147 has not yet been referenced specifically in any publications.
Publishing research using ab117147 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"