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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 (H3 (methyl K4)) plays an important role in epigenetic regulation of genes. It has been well demonstrated that H3 (methyl-K4) leads to chromatin gene activation in many organisms. H3 (di-methyl K4) may serve as global epigenertic mark in euchromatin. Thus, identification of genes activated through H3 (methyl-K4) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.
EpiSeeker ChIP Kit - Histone H3 (methyl K4)-Tissue (ab117145) enables the user to investigate in vivo H3 (methyl K4)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
ab117145 uses an antibody that detects Histone H3 di-methylated on K4.
If you are using cells (adherent or suspension) as starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143)
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Dimethyl-H3-K4, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||24 tests||48 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-Dimethyl-H3-K4 (1 mg/ml)||1 x 25µl||1 x 50µl|
|ECM1 (Wash Buffer)||1 x 28ml||2 x 28ml|
|ECM2 (Antibody Buffer)||1 x 15ml||1 x 30ml|
|ECM3 (Lysis Buffer)||1 x 4ml||1 x 6ml|
|ECM4 (ChIP Dilution Buffer)||1 x 4ml||1 x 6ml|
|ECM5 (DNA Release Buffer)||1 x 1ml||1 x 2ml|
|ECM6 (Reverse Buffer)||1 x 1ml||1 x 2ml|
|ECM7 (Binding Buffer)||1 x 5ml||1 x 8ml|
|ECM8 (Elution Buffer)||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Homogenizing Buffer||1 x 5ml||1 x 10ml|
|Normal Mouse IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Protease Inhibitor Cocktail (100X)||1 x 25µl||1 x 40µl|
|Proteinase K (10 mg/ml)||1 x 25µl||1 x 50µl|
Our Abpromise guarantee covers the use of ab117145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
ab117145 has not yet been referenced specifically in any publications.
Publishing research using ab117145 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"