EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143)

Overview

  • Product nameEpiSeeker ChIP Kit - Histone H3 (methyl K4)See all Histone H3 (K4 methylation) kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    Methylation of histone H3 at lysine 4 (H3 (methyl K4)) plays an important role in epigenetic regulation of genes. It has been well demonstrated that H3 (methyl-K4) leads to chromatin gene activation in many organisms. H3 (di-methyl K4) may serve as global epigenertic mark in euchromatin. Thus, identification of genes activated through H3 (methyl-K4) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.

    EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143) enables the user to investigate in vivo H3 (methyl K4)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

    ab117143 uses an antibody that detects Histone H3 di-methylated on K4.

    If you are using tissue as starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (methyl K4) - Tissue (ab117145)

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Dimethyl-H3-K4, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit can be stable for up to 6 months from the shipment date when stored properly.

  • Tested applicationsChIPmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-Dimethyl-H3-K4 (1 mg/ml) 1 x 25µl 1 x 50µl
    ECM1 (Wash Buffer) 1 x 28ml 2 x 28ml
    ECM2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    ECM3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml
    ECM3B (Lysis Buffer) 1 x 2ml 2 x 2ml
    ECM4 (ChIP Dilution Buffer) 1 x 2ml 2 x 2ml
    ECM5 (DNA Release Buffer) 1 x 1ml 1 x 2ml
    ECM6 (Reverse Buffer) 1 x 1ml 1 x 2ml
    ECM7 (Binding Buffer) 1 x 5ml 1 x 8ml
    ECM8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Protease Inhibitor Cocktail (100X) 1 x 25µl 1 x 40µl
    Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117143 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ChIP Use at an assay dependent concentration.

    EpiSeeker ChIP Kit - Histone H3 (methyl K4) images

    • Chromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECM4) and 2mg of ab117143 (blue). 2µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

    Protocols

    References for EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143)

    ab117143 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab117143.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"