EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143)
- Product nameEpiSeeker ChIP Kit - Histone H3 (methyl K4)See all Histone H3 (K4 methylation) kits ...
- Sample typeAdherent cells, Suspension cells
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human
- Product overview
Methylation of histone H3 at lysine 4 (H3 (methyl K4)) plays an important role in epigenetic regulation of genes. It has been well demonstrated that H3 (methyl-K4) leads to chromatin gene activation in many organisms. H3 (di-methyl K4) may serve as global epigenertic mark in euchromatin. Thus, identification of genes activated through H3 (methyl-K4) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.
EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143) enables the user to investigate in vivo H3 (methyl K4)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
ab117143 uses an antibody that detects Histone H3 di-methylated on K4.
If you are using tissue as starting sample material, we recommend the use of EpiSeeker ChIP Kit - Histone H3 (methyl K4) - Tissue (ab117145)
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Dimethyl-H3-K4, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit can be stable for up to 6 months from the shipment date when stored properly.
- Tested applicationsChIP more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-Dimethyl-H3-K4 (1 mg/ml) 1 x 25µl 1 x 50µl ECM1 (Wash Buffer) 1 x 28ml 2 x 28ml ECM2 (Antibody Buffer) 1 x 15ml 1 x 30ml ECM3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml ECM3B (Lysis Buffer) 1 x 2ml 2 x 2ml ECM4 (ChIP Dilution Buffer) 1 x 2ml 2 x 2ml ECM5 (DNA Release Buffer) 1 x 1ml 1 x 2ml ECM6 (Reverse Buffer) 1 x 1ml 1 x 2ml ECM7 (Binding Buffer) 1 x 5ml 1 x 8ml ECM8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl Protease Inhibitor Cocktail (100X) 1 x 25µl 1 x 40µl Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
- Research Areas
Our Abpromise guarantee covers the use of ab117143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker ChIP Kit - Histone H3 (methyl K4) images
Chromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECM4) and 2mg of ab117143 (blue). 2µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
References for EpiSeeker ChIP Kit - Histone H3 (methyl K4) (ab117143)
ab117143 has not yet been referenced specifically in any publications.