EpiSeeker ChIP Kit - Histone H3 (tri-methyl K9) (ab117142)


  • Product nameEpiSeeker ChIP Kit - Histone H3 (tri-methyl K9)
    See all Histone H3 (K9 methylation) kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    The position of the histone H3 at lysine 9 (H3 (K9)) is important for epigenetic regulation of genes, as it can be either methylated or acetylated. It has been demonstrated that switching H3(acetyl K9) to methylation on H3 (methyl K9) leads to chromatin gene silencing in many organisms. H3 (tri-methyl K9) is a stable heterochromatin mark which promotes gene silencing. Thus, identification of genes silenced through H3 (tri-methyl K9) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.

    EpiSeeker ChIP Kit - Histone H3 (tri-methyl K9) (ab117142) enables the user to investigate in vivo H3 (tri-methyl K9)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Tri-Methyl-H3-K9, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit can be stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsChIPmore details


  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    100X Protease Inhibitor Cocktail 1 x 25µl 1 x 40µl
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-Tri-Methyl-H3-K9 (1 mg/ml) 1 x 25µl 1 x 50µl
    ECM1 (Wash Buffer) 1 x 28ml 2 x 28ml
    ECM2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    ECM3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml
    ECM3B (Lysis Buffer) 1 x 2ml 2 x 2ml
    ECM4 (ChIP Dilution Buffer) 1 x 2ml 2 x 2ml
    ECM5 (DNA Release Buffer) 1 x 1ml 1 x 2ml
    ECM6 (Reverse Buffer) 1 x 1ml 1 x 2ml
    ECM7 (Binding Buffer) 1 x 5ml 1 x 10ml
    ECM8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl
  • Research Areas


    Our Abpromise guarantee covers the use of ab117142 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ChIP Use at an assay dependent concentration.

    EpiSeeker ChIP Kit - Histone H3 (tri-methyl K9) images

    • Chromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECM4) and 1mg of ab117142 (blue). 1µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.


    References for EpiSeeker ChIP Kit - Histone H3 (tri-methyl K9) (ab117142)

    ab117142 has not yet been referenced specifically in any publications.

    Product Wall

    The host species of the antibody (in other words, the species the antibody was created in) in the histone H3 (tri-methyl K9) ChIP kit ab117142 is mouse.

    Thank you very much for your call yesterday and for your questions about our ChIP kit, ab117142.

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    The protocol of ab117142 kit can be slightly modified for using intissue ChIP.

    1). Place the tissue sample into a 60 or 100 mm plate. Remove unwanted
    tissue such as fat and necrotic material from the sample. Weigh the sample
    and cu...

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