EpiSeeker ChIP Kit - Histone H4 (acetyl) - Tissue (ab117151)

Overview

  • Product nameEpiSeeker ChIP Kit - Histone H4 (acetyl) - TissueSee all Histone H4 acetylation kits ...
  • Sample type
    Tissue
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.

    EpiSeeker ChIP Kit - Histone H4 (acetyl)-Tissue (ab117151) enables the user to investigate in vivo H4 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

    If you are using cells (adherent or in suspension) as starting material, we recommend the use of EpiSeeker ChIP Kit - Histone H4 (acetyl) (ab117149)

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H4, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsChIPmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab117151 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ChIP Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker ChIP Kit - Histone H4 (acetyl) - Tissue (ab117151)

    ab117151 has not yet been referenced specifically in any publications.

    Product Wall

    H3-K9 and K14 will be recognized by the antibody in ab117150 and H4-K5/8/12/16 will be recognized by antibody in ab117151. I hope this is helpful. Please contact me again if you have any further questions.

    I am sorry for the delay in our reply. Here are answers to your questions: 1. The antibodies included in ab117136, 117144, 117145, 117147, 117150 and117151 are not sold separately in our catalog. 2. The antibodies in kits ab117144, 117145 and117147 hav...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"