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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
The EpiSeeker ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of EpiSeeker Chromatin Extraction Kit (ab117152) for sample preparation.
Store ECP1, Non-Immune IgG, Anti-RNA Polymerase II, Proteinase K, GAPDH Primer – Forward, GADPH Primer – Reverse, and 8-Well Assay Strips (With 1 Frame) at 4°C away from light.
Store remaining components at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|8-Well Strip Caps||6 units||12 units|
|96-Well PCR Plate||1 unit||1 unit|
|Adhesive Covering Film||1 unit||1 unit|
|Anti-RNA Polymerase II (1 mg/ml)||1 x 8µl||1 x 16µl|
|ChIP Buffer||1 x 6ml||1 x 12ml|
|DNA Release Buffer||ECP3||1 x 14ml||1 x 28ml|
|GAPDH Primer - Forward (20 µM)||1 x 8µl||1 x 16µl|
|GAPDH Primer - Reverse (20 µM)||1 x 8µl||1 x 16µl|
|Non-Immune IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Proteinase K (10 mg/ml)||1 x 55µl||1 x 110µl|
|Sonication Frame||1 unit||1 unit|
Our Abpromise guarantee covers the use of ab117138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.
Publishing research using ab117138 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"