EpiSeeker ChIP Kit - One Step (ab117138)
- Product nameEpiSeeker ChIP Kit - One StepSee all ChIP Kit kits ...
- Assay time5h 00m
- Species reactivityReacts with: Human
Predicted to work with: Mouse, all Mammals
- Product overview
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
The EpiSeeker ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of EpiSeeker Chromatin Extraction Kit (ab117152) for sample preparation.
Store ECP1, Non-Immune IgG, Anti-RNA Polymerase II, Proteinase K, GAPDH Primer – Forward, GADPH Primer – Reverse, and 8-Well Assay Strips (With 1 Frame) at 4°C away from light.
Store remaining components at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsChIP more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 48 tests 96 tests 10X Wash Buffer ECP1 1 x 10ml 1 x 20ml 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Strip Caps 6 units 12 units 96-Well PCR Plate 1 unit 1 unit Adhesive Covering Film 1 unit 1 unit Anti-RNA Polymerase II (1 mg/ml) 1 x 8µl 1 x 16µl ChIP Buffer ECP2 1 x 6ml 1 x 12ml DNA Release Buffer ECP3 1 x 14ml 1 x 28ml GAPDH Primer - Forward (20 µM) 1 x 8µl 1 x 16µl GAPDH Primer - Reverse (20 µM) 1 x 8µl 1 x 16µl Non-Immune IgG (1 mg/ml) 1 x 10µl 1 x 20µl Proteinase K (10 mg/ml) 1 x 55µl 1 x 110µl Sonication Frame 1 unit 1 unit
- Research Areas
Our Abpromise guarantee covers the use of ab117138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker ChIP Kit - One Step images
Chromatin was prepared from HeLa cells following Abcam's X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with ab8580 (Histone H3 tri methyl K4) using ab117138. No antibody was added to the blank (yellow).
The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.
The analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by the EpiSeeker ChIP Kit - One Step, with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.
References for EpiSeeker ChIP Kit - One Step (ab117138)
This product has been referenced in:
- Onyeagucha BC et al. S100P/RAGE signaling regulates microRNA-155 expression via AP-1 activation in colon cancer. Exp Cell Res 319:2081-90 (2013). ChIP ; Human . Read more (PubMed: 23693020) »