EpiSeeker Chromatin Extraction Kit (ab117152)
- Product nameEpiSeeker Chromatin Extraction KitSee all Chromatin Extraction kits ...
- Tests1 x 100 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time1h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
EpiSeeker Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Abcam’s one-hour ChIP method using the EpiSeeker ChIP Kit - One Step (ab117138). The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Not sure if this is what you need? Check our EpiSeeker Sample Preparation Guide.
Store Protease Inhibitor cocktails at 4°C.
Store remaining components at room temperature.
All components of the kit are stable for 6 months from the date of
shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Components 100 tests ECE1 (10X Lysis Buffer) 1 x 11ml ECE2 (Extraction Buffer) 1 x 11ml ECE3 (Chromatin Buffer) 1 x 11ml Protease Inhibitor Cocktail (1000X) 1 x 110µl
- Research Areas
Our Abpromise guarantee covers the use of ab117152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Chromatin Extraction Kit images
ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments Image courtesy of anonymous Abreview
References for EpiSeeker Chromatin Extraction Kit (ab117152)
ab117152 has not yet been referenced specifically in any publications.