Overview
- Product nameEpiSeeker Chromatin Extraction KitSee all Chromatin Extraction kits ...
- Tests1 x 100 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time1h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
EpiSeeker Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Abcam’s one-hour ChIP method using the EpiSeeker ChIP Kit - One Step (ab117138). The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Not sure if this is what you need? Check our EpiSeeker Sample Preparation Guide.
- Notes
Store Protease Inhibitor cocktails at 4°C.
Store remaining components at room temperature.
All components of the kit are stable for 6 months from the date of
shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
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Components 100 tests ECE1 (10X Lysis Buffer) 1 x 11ml ECE2 (Extraction Buffer) 1 x 11ml ECE3 (Chromatin Buffer) 1 x 11ml Protease Inhibitor Cocktail (1000X) 1 x 110µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Chromatin Extraction Kit images
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Functional Studies - EpiSeeker Chromatin Extraction kit (ab117152)ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152. -
EpiSeeker Chromatin Extraction Kit (ab117152)Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments Image courtesy of anonymous Abreview
Protocols
References for EpiSeeker Chromatin Extraction Kit (ab117152)
ab117152 has not yet been referenced specifically in any publications.
