EpiSeeker DNA-Protein Binding Assay Kit (Fluorometric) (ab117140)
Overview
- Product nameEpiSeeker DNA-Protein Binding Assay Kit (Fluorometric)See all DNA-protein kits ...
- Tests1 x 96 test
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes.
EpiSeeker DNA-Protein Binding Assay Kit (Fluorometric) (ab117140) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.
- Notes
Store EDP3 and EDP6 at –20°C
Store EDP1, DTT and 8-Well Assay Strips at 4°C
Store all other components at room temperature.
All components of the kit are stable for 6 months from the date of shipment, when properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components Identifier 96 tests 10X Wash Buffer EDP1 1 x 20ml 8-Well Assay Strips (with Frame) 12 units 96-Well Assay Plate 1 unit Antibody Buffer EDP2 1 x 6ml Assay Buffer EDP4 1 x 10ml DTT (500X) 1 x 22µl Extract Cleaner EDP3 1 x 100µl Fluoro Developer EDP6 1 x 0.2ml Oligo Release Solution EDP5 1 x 11ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker DNA-Protein Binding Assay Kit (Fluorometric) (ab117140)
ab117140 has not yet been referenced specifically in any publications.
