EpiSeeker DNA demethylase (total) Activity Quantification Assay Kit (ab113472)
Overview
- Product nameEpiSeeker DNA demethylase (total) Activity Quantification Assay KitSee all DNA demethylase kits ...
- Assay typeEnzyme activity
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.
EpiSeeker DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.
ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.
- Notes
Store EDD3, EDD4 and EDD6 at –20°C.
Store EDD1, EDD5, EDD7, and 8-Well Assay Strips at 4°C away from light.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EDD1 (10X Wash Buffer) 1 x 11ml 1 x 22ml EDD2 (Demethylase Assay Buffer) 1 x 3ml 1 x 6ml EDD3 (Demethylase Substrate) 1 x 0.1ml 1 x 0.2ml EDD4 (Demethylation Standard, 10 µg/ml) 1 x 20µl 1 x 40µl EDD5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 8µl EDD6 (Detecting Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl EDD7 (Developing Solution) 1 x 5ml 1 x 10ml EDD8 (Stop Solution) 1 x 3ml 1 x 6ml Substrate Binding Solution 1 x 5ml 1 x 10ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker DNA demethylase (total) Activity Quantification Assay Kit images
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Functional Studies - EpiSeeker DNA demethylase (total) Activity Quantification Assay Kit (ab113472)Nuclear extracts were prepared from MCF-7 cells using the EpiSeeker Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.
Protocols
References for EpiSeeker DNA demethylase (total) Activity Quantification Assay Kit (ab113472)
ab113472 has not yet been referenced specifically in any publications.
