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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467) allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
Store EDM3, EDM4, EDM6, and EDM7 at –20°C away from light.
Store EDM1, EDM5, EDM8, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EDM2, EDM9, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|EDM1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EDM2 (DNMT Assay Buffer)||1 x 4ml||1 x 8ml|
|EDM3 (Adomet, 50X)||1 x 60µl||1 x 120µl|
|EDM4 (DNMT Enzyme Control, 50 µg/ml)||1 x 6µl||1 x 12µl|
|EDM5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EDM6 (Detection Antibody, 400 µg/ml)||1 x 6µl||1 x 12µl|
|EDM8 (Developer Solution)||1 x 5ml||1 x 10ml|
|EDM9 (Stop Solution)||1 x 5ml||1 x 10ml|
|Enhancer Solution||1 x 6µl||1 x 12µl|
Our Abpromise guarantee covers the use of ab113467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113467 has not yet been referenced specifically in any publications.
Publishing research using ab113467 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"