EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467)
Overview
- Product nameEpiSeeker DNMT Activity Quantification Kit (Colorimetric)See all DNMT kits ...
- Assay typeQuantitative
- Assay time3h 45m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Fungi - Product overview
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467) allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
- Notes
Store EDM3, EDM4, EDM6, and EDM7 at –20°C away from light.
Store EDM1, EDM5, EDM8, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EDM2, EDM9, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit EDM1 (10X Wash Buffer) 1 x 14ml 1 x 28ml EDM2 (DNMT Assay Buffer) 1 x 4ml 1 x 8ml EDM3 (Adomet, 50X) 1 x 60µl 1 x 120µl EDM4 (DNMT Enzyme Control, 50 µg/ml) 1 x 6µl 1 x 12µl EDM5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EDM6 (Detection Antibody, 400 µg/ml) 1 x 6µl 1 x 12µl EDM8 (Developer Solution) 1 x 5ml 1 x 10ml EDM9 (Stop Solution) 1 x 5ml 1 x 10ml Enhancer Solution 1 x 6µl 1 x 12µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker DNMT Activity Quantification Kit (Colorimetric) images
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Functional Studies - EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467)Demonstration of high sensitivity and specificity of the DNMT activity assay achieved by using nuclear extracts with ab113467. Nuclear extracts were prepared from MCF-7 cells using ab113474. -
Functional Studies - EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467)Demonstration of high sensitivity and specificity of the DNMT activity/inhibition assay achieved by using recombinant DNMT1 with ab113467.
Protocols
References for EpiSeeker DNMT Activity Quantification Kit (Colorimetric) (ab113467)
ab113467 has not yet been referenced specifically in any publications.
