EpiSeeker DNMT Activity Quantification Kit (Fluorometric) (ab113468)
Overview
- Product nameEpiSeeker DNMT Activity Quantification Kit (Fluorometric)See all DNMT kits ...
- Assay time3h 45m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Fungi - Product overview
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
EpiSeeker DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment..
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- Notes
Store EDM3, EDM4, EDM6, EDM7 and EDM8 at –20°C away from light.
Store EDM1, EDM5, EDM9, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EDM2, EDM10, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit EDM1 (10X Wash Buffer) 1 x 14ml 1 x 28ml EDM10 (Fluoro Dilutor) 1 x 4ml 1 x 8ml EDM2 (DNMT Assay Buffer) 1 x 4ml 1 x 8ml EDM3 (Adomet, 50X) 1 x 60µl 1 x 120µl EDM4 (DNMT Enzyme Control, 50 µg/ml) 1 x 6µl 1 x 12µl EDM5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EDM6 (Detection Antibody, 400 µg/ml) 1 x 6µl 1 x 12µl EDM7 (Enhancer Solution) 1 x 6µl 1 x 12µl EDM8 (Fluoro Developer) 1 x 6µl 1 x 12µl EDM9 (Fluoro Enhancer) 1 x 6µl 1 x 12µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker DNMT Activity Quantification Kit (Fluorometric) images
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Functional Studies - EpiSeeker DNMT Activity Quantification Kit (Fluorometric) (ab113468)Demonstration of high sensitivity and specificity achieved by using recombinant DNMT1 with ab113468.
Protocols
References for EpiSeeker DNMT Activity Quantification Kit (Fluorometric) (ab113468)
ab113468 has not yet been referenced specifically in any publications.
