Overview

  • Product nameEpiSeeker DNMT1 Assay KitSee all DNMT1 kits ...
  • Tests
    1 x 24 test
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.

    EpiSeeker DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

    Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.

  • Notes

    Store EDN3 and EDN5 at –20°C away from light.

    Store EDN1, EDN4, EDN6, END8, Blocking Buffer and the 8-Well Assay Strips at 4°C away from light.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 96 tests 48 tests
    8-Well Assay Strips (with Frame) 12 units 6 units
    BB (Blocking Buffer) 1 x 20ml 1 x 10ml
    EDN1 (10X Wash Buffer) 1 x 28ml 1 x 14ml
    EDN2 (Assay Buffer) 1 x 10ml 1 x 5ml
    EDN3 (DNMT1 Standard, 20 µg/ml) 1 x 40µl 1 x 20µl
    EDN4 (Affinity Antibody, 500 µg/ml) 1 x 16µl 1 x 8µl
    EDN5 (Detection Antibody, 200 µg/ml) 1 x 20µl 1 x 10µl
    EDN6 (Developing Solution) 1 x 12ml 1 x 6ml
    EDN7 (Stop Solution) 1 x 11ml 1 x 6ml
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab113469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker DNMT1 Assay Kit images

    • Nuclear extracts were prepared from MCF-7 cells using the EpiSeeker Nuclear Extraction Kit (ab113474) and DNMT1 activity was measured using ab113469. DNMT3A and DNMT3B activities were measured using ab113470 EpiSeeker DNMT3A Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.

    Protocols

    References for EpiSeeker DNMT1 Assay Kit (ab113469)

    ab113469 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113469.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"