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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
EpiSeeker DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
Store EDN3 and EDN5 at –20°C away from light.
Store EDN1, EDN4, EDN6, END8, Blocking Buffer and the 8-Well Assay Strips at 4°C away from light.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||96 tests||48 tests|
|8-Well Assay Strips (with Frame)||12 units||6 units|
|BB (Blocking Buffer)||1 x 20ml||1 x 10ml|
|EDN1 (10X Wash Buffer)||1 x 28ml||1 x 14ml|
|EDN2 (Assay Buffer)||1 x 10ml||1 x 5ml|
|EDN3 (DNMT1 Standard, 20 µg/ml)||1 x 40µl||1 x 20µl|
|EDN4 (Affinity Antibody, 500 µg/ml)||1 x 16µl||1 x 8µl|
|EDN5 (Detection Antibody, 200 µg/ml)||1 x 20µl||1 x 10µl|
|EDN6 (Developing Solution)||1 x 12ml||1 x 6ml|
|EDN7 (Stop Solution)||1 x 11ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113469 has not yet been referenced specifically in any publications.
Publishing research using ab113469 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"