Overview
- Product nameEpiSeeker DNMT1 Assay KitSee all DNMT1 kits ...
- Tests1 x 24 test
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
EpiSeeker DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
- Notes
Store EDN3 and EDN5 at –20°C away from light.
Store EDN1, EDN4, EDN6, END8, Blocking Buffer and the 8-Well Assay Strips at 4°C away from light.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 48 tests 8-Well Assay Strips (with Frame) 12 units 6 units BB (Blocking Buffer) 1 x 20ml BB (Blocking Buffer) 1 x 20ml 1 x 10ml 1 x 10ml EDN1 (10X Wash Buffer) 1 x 28ml 1 x 14ml EDN2 (Assay Buffer) 1 x 10ml 1 x 5ml EDN3 (DNMT1 Standard, 10 µg/ml) 1 x 60µl 1 x 30µl EDN4 (Affinity Antibody, 100 µg/ml) 1 x 16µl 1 x 8µl EDN5 (Detection Antibody, 200 µg/ml) 1 x 20µl 1 x 10µl EDN6 (Developing Solution) 1 x 12ml 1 x 6ml EDN7 (Stop Solution) 1 x 11ml 1 x 6ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker DNMT1 Assay Kit images
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Functional Studies - EpiSeeker DNMT1 Assay Kit (ab113469)Nuclear extracts were prepared from MCF-7 cells using the EpiSeeker Nuclear Extraction Kit (ab113474) and DNMT1 activity was measured using ab113469. DNMT3A and DNMT3B activities were measured using ab113470 EpiSeeker DNMT3A Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.
Protocols
References for EpiSeeker DNMT1 Assay Kit (ab113469)
ab113469 has not yet been referenced specifically in any publications.
