EpiSeeker DNMT1 Inhibitor Screening Assay Kit (ab113465)
- Product nameEpiSeeker DNMT1 Inhibitor Screening Assay KitSee all DNMT1 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase. The selective inhibition of DNMT1 may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT1 inhibitors could be a new addition to cancer therapeutic agents.
EpiSeeker DNMT1 Inhibitor Screening Assay Kit (ab113465) allows the user to selectively screen DNMT1 inhibitors quickly. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
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Store EMC3 and EMC5, at –20°C away from light.
Store EMC1, EMC2, EMC4, EMC6 and 8-Well Substrate-Coated Strips at 4°C.
Store EMC7 at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components Identifier 48 tests 96 tests 8-well Substrate-Coated Strip (with frame) — 12 units EMC1 (10X Wash Buffer) — 1 x 22ml EMC2 (DNMT Assay Buffer) — 1 x 4ml EMC3 (Adomet) — 1 x 70µl EMC4 (Capture Antibody) — 1 x 10µl EMC5 (Detection Antibody) — 1 x 20µl EMC6 (Developing Solution) — 1 x 12ml EMC7 (Stop Solution) — 1 x 6ml Enhancer Solution — 1 x 6µl
- Research Areas
Our Abpromise guarantee covers the use of ab113465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker DNMT1 Inhibitor Screening Assay Kit images
Recombinant DNMT1 was incubated with substrate and inhibitors. DNMT1 activity was measured using ab113465 in the presence or absence of inhibitors.
References for EpiSeeker DNMT1 Inhibitor Screening Assay Kit (ab113465)
ab113465 has not yet been referenced specifically in any publications.