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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase. The selective inhibition of DNMT1 may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT1 inhibitors could be a new addition to cancer therapeutic agents.
EpiSeeker DNMT1 Inhibitor Screening Assay Kit (ab113465) allows the user to selectively screen DNMT1 inhibitors quickly. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
Store EMC3 and EMC5, at –20°C away from light.
Store EMC1, EMC2, EMC4, EMC6 and 8-Well Substrate-Coated Strips at 4°C.
Store EMC7 at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-well Substrate-Coated Strip (with frame)||6 units||12 units|
|EMC1 (10X Wash Buffer)||1 x 11ml||1 x 22ml|
|EMC2 (DNMT Assay Buffer)||1 x 2ml||1 x 4ml|
|EMC3 (Adomet)||1 x 35µl||1 x 70µl|
|EMC4 (Capture Antibody)||1 x 5µl||1 x 8µl|
|EMC5 (Detection Antibody)||1 x 10µl||1 x 20µl|
|EMC6 (Developing Solution)||1 x 6ml||1 x 12ml|
|EMC7 (Stop Solution)||1 x 3ml||1 x 6ml|
|Enhancer Solution||1 x 12µl||1 x 6µl|
Our Abpromise guarantee covers the use of ab113465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113465 has not yet been referenced specifically in any publications.
Publishing research using ab113465 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"