Overview
- Product nameEpiSeeker DNMT3A Assay Kit
- Tests1 x 24 test
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development.
EpiSeeker DNMT3A Assay Kit (ab113470) allows the user to quickly and consistently measure the amount of DNMT3A. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3A assay experiment. ab113470 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units BB (Blocking Buffer) 1 x 10ml 1 x 20ml EDA1 (10X Wash Buffer) 1 x 12ml 1 x 25ml EDA2 (Assay Buffer) 1 x 5ml 1 x 10ml EDA3 (DNMT3A Standard, 20 µg/ml) 1 x 16µl 1 x 30µl EDA4 (Affinity Antibody, 100 µg/ml) 1 x 8µl 1 x 16µl EDA5 (Detection Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl EDA6 (Developing Solution) 1 x 6ml 1 x 12ml EDA7 (Stop Solution) 1 x 6ml 1 x 11ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker DNMT3A Assay Kit images
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Functional Studies - EpiSeeker DNMT3A Assay Kit (ab113470)Nuclear extracts were prepared from MCF-7 cells using the EpiSeeker Nuclear Extraction Kit (ab113474) and DNMT3A activity was measured using ab113470. DNMT1 and DNMT3B activities were measured using ab113469 EpiSeeker DNMT1 Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.
Protocols
References for EpiSeeker DNMT3A Assay Kit (ab113470)
ab113470 has not yet been referenced specifically in any publications.
