Overview

  • Product nameEpiSeeker DNMT3A Assay Kit
  • Tests
    1 x 24 test
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development.

     

    EpiSeeker DNMT3A Assay Kit (ab113470) allows the user to quickly and consistently measure the amount of DNMT3A. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3A assay experiment. ab113470 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

    Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 48 tests 96 tests
    8-Well Assay Strips (with Frame) 6 units 12 units
    BB (Blocking Buffer) 1 x 10ml 1 x 20ml
    EDA1 (10X Wash Buffer) 1 x 12ml 1 x 25ml
    EDA2 (Assay Buffer) 1 x 5ml 1 x 10ml
    EDA3 (DNMT3A Standard, 20 µg/ml) 1 x 16µl 1 x 30µl
    EDA4 (Affinity Antibody, 100 µg/ml) 1 x 8µl 1 x 16µl
    EDA5 (Detection Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl
    EDA6 (Developing Solution) 1 x 6ml 1 x 12ml
    EDA7 (Stop Solution) 1 x 6ml 1 x 11ml
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab113470 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker DNMT3A Assay Kit images

    • Nuclear extracts were prepared from MCF-7 cells using the EpiSeeker Nuclear Extraction Kit (ab113474) and DNMT3A activity was measured using ab113470. DNMT1 and DNMT3B activities were measured using ab113469 EpiSeeker DNMT1 Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.

    Protocols

    References for EpiSeeker DNMT3A Assay Kit (ab113470)

    ab113470 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113470.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"