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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development. The selective inhibition of DNMT3B may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT3B inhibitors could be a new addition to cancer therapeutic agents.
EpiSeeker DNMT3B Inhibitor Screening Assay Kit (ab113466) allows the user to selectively screen DNMT3B activity/inhibitors in an ELISA-like format. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.
Store EMH3 and EMH5 at –20°C away from light.
Store EMH1, EMH2, EMH4, EMH6 and the 8-Well Substrate-Coated Strips at 4°C away from light.
Store EMH7 at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-well Substrate-Coated Strip (with frame)||6 units||12 units|
|EMH1 (10X Wash Buffer)||1 x 11ml||1 x 22ml|
|EMH2 (DNMT Assay Buffer)||1 x 2ml||1 x 4ml|
|EMH3 (Adomet, 8 mM)||1 x 35µl||1 x 70µl|
|EMH4 (Capture Antibody)||1 x 5µl||1 x 10µl|
|EMH5 (Detection Antibody)||1 x 10µl||1 x 20µl|
|EMH6 (Developing Solution)||1 x 6ml||1 x 12ml|
|EMH7 (Stop Solution)||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113466 has not yet been referenced specifically in any publications.
Publishing research using ab113466 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"