Overview
- Product nameEpiSeeker Global Protein Sumoylation Assay Kit
- Sample typeCell culture extracts, Tissue Extracts
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: all Mammals - Product overview
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Thus, detection of in vivo protein sumoylation (SUMO conjugation) would provide useful information for understanding SUMO modification as a regulatory control mechanisms for many nuclear proteins.
EpiSeeker Global Protein Sumoylation Assay Kit (ab115131) allows the user to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues extracts.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Control Strips 2 units 3 units ESS1 (10X Wash Buffer) 1 x 25ml 2 x 25ml ESS10 (Stop Solution) 1 x 3ml 1 x 6ml ESS2 (Binding Buffer) 1 x 15ml 1 x 30ml ESS3 (Negative Control, 1 µg/µl) 1 x 120µl 1 x 240µl ESS4 (Blocking Buffer) 1 x 10ml 1 x 20ml ESS5 (Sumo Assay Buffer) 1 x 2ml 1 x 4ml ESS6 (Sumo Protein, 1 µg/µl) 1 x 10µl 1 x 20µl ESS7 (Sumo Antibody, 0.5 µg/µl) 1 x 20µl 1 x 40µl ESS8 (Signal Report Solution) 1 x 5µl 1 x 10µl ESS9 (Color Development Solution) 1 x 6ml 1 x 12ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Global Protein Sumoylation Assay Kit (ab115131)
ab115131 has not yet been referenced specifically in any publications.
